Objective: To study the effectiveness of human tubal Estromal Stem Cells (htESCs) therapy by intra-nasal or intra-peritoneal route in experimental cigarette smoke-induced emphysema in C57BL/6 mice. Design: To achieve that, C57BL/6 mice were exposed to cigarette smoke for 75 days (2 times / day) and received or not two doses of htMSCS either intraperitoneally or intranasally. Experiments were performed on day 76th. Materials and Methods: Briefly, brochoalveolar lavage fluid was obtained and either used to measure cytokine secretion or inflammatory infiltrate. Moreover, lung tissue was also submitted to imunohistochemistry for the transcription factors NF-AT and NF-kB and also IL-10. Results: Our results clearly show that htMSCs greatly reduced lung inflammation, with decreased cellular infiltrate of T CD4 and T CD8 lymphocytes, which was confirmed by reduced NF-AT staining in lung tissues. This was associated with reduced pro-inflammatory cytokine secretion, as TNF-a, IL-1b and IL-6, less mucus and collagen deposition, and interestingly, increased IL-10. Conclusion: Altogether, our data indicate that therapy with htMSCs for the treatment of emphysematous disease may be a promising clinical approach. Moreover, intranasal route is also effective, and must taken in consideration due to its lack of invasiveness.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.