Skeletal muscle atrophy is the consequence of protein degradation exceeding protein synthesis. This arises for a multitude of reasons including the unloading of muscle during microgravity, post-surgery bedrest, immobilization of a limb after injury, and overall disuse of the musculature. The development of therapies prior to skeletal muscle atrophy settings to diminish protein degradation is scarce. Mitochondrial dysfunction is associated with skeletal muscle atrophy and contributes to the induction of protein degradation and cell apoptosis through increased levels of ROS observed with the loss of organelle function. ROS binds mtDNA, leading to its degradation and decreasing functionality. Mitochondrial transcription factor A (TFAM) will bind and coat mtDNA, protecting it from ROS and degradation while increasing mitochondrial function. Exercise stimulates cell signaling pathways that converge on and increase PGC-1α, a well-known activator of the transcription of TFAM and mitochondrial biogenesis. Therefore, in the present review we are proposing, separately, exercise and TFAM treatments prior to atrophic settings (muscle unloading or disuse) alleviate skeletal muscle atrophy through enhanced mitochondrial adaptations and function. Additionally, we hypothesize the combination of exercise and TFAM leads to a synergistic effect in targeting mitochondrial function to prevent skeletal muscle atrophy.

Although hyperhomocysteinemia (HHcy) occurs due to the deficiency in cystathionine-β-synthase (CBS) causing skeletal muscle dysfunction, it is still unclear whether this effect is mediated through oxidative/endoplasmic reticulum (ER)-stress or both. Nevertheless, there is no treatment option available to improve HHcy-mediated muscle injury. Hydrogen sulfide (HS) is an anti-oxidant compound and patients with CBS mutation do not produce HS. In this study, we hypothesized that HS mitigates HHcy-induced redox imbalance/ER-stress during skeletal muscle atrophy via JNK-phosphorylation. We used CBS mice to study HHcy-mediated muscle atrophy and treated them with sodium hydrogen sulfide (NaHS, an HS donor). Proteins and mRNAs were examined by Western blots and qPCR. Pro-inflammatory cytokines were also measured. Muscle mass and strength were studied via fatigue-susceptibility test. Our data revealed that HHcy was detrimental to skeletal mass, particularly gastrocnemius and quadriceps muscles weights. We noticed that oxidative-stress were reversed by NaHS in Hcy-treated C2C12 cells. Interestingly, ER-stress markers (GRP78, ATF6, pIRE1α, and pJNK) were elevated in-vivo and in-vitro, and NaHS mitigated these effects. Additionally, we observed that JNK-phosphorylation was upregulated in C2C12 after Hcy treatment, but NaHS could not reduce this effect. Furthermore, inflammatory cytokines IL-6 and TNF-α were higher in plasma from CBS as compared to wild-type mice. FOXO1-mediated Atrogin-1 and MuRF-1 upregulation were attenuated by NaHS. Functional studies revealed that NaHS administration improved muscle fatigability in CBS mice. In conclusion, our work provides evidence that NaHS is beneficial in mitigating HHcy-mediated skeletal injury incited by oxidative/ER-stress responses.

The aim of the present study was to investigate whether short-term, concurrent exercise training before hindlimb suspension (HLS) prevents or diminishes both soleus and gastrocnemius atrophy and to analyze whether changes in mitochondrial molecular markers were associated. Male C57BL/6 mice were assigned to control at 13 ± 1 wk of age, 7-day HLS at 12 ± 1 wk of age (HLS), 2 wk of exercise training before 7-day HLS at 10 ± 1 wk of age (Ex+HLS), and 2 wk of exercise training at 11 ± 1 wk of age (Ex) groups. HLS resulted in a 27.1% and 21.5% decrease in soleus and gastrocnemius muscle weight-to-body weight ratio, respectively. Exercise training before HLS resulted in a 5.6% and 8.1% decrease in soleus and gastrocnemius weight-to-body weight ratio, respectively. Exercise increased mitochondrial biogenesis- and function-associated markers and slow myosin heavy chain (SMHC) expression, and reduced fiber-type transitioning marker myosin heavy chain 4 (Myh4). Ex+HLS revealed decreased reactive oxygen species (ROS) and oxidative stress compared with HLS. Our data indicated the time before an atrophic setting, particularly caused by muscle unloading, may be a useful period to intervene short-term, progressive exercise training to prevent skeletal muscle atrophy and is associated with mitochondrial biogenesis, function, and redox balance.NEW & NOTEWORTHY Mitochondrial dysfunction is associated with disuse-induced skeletal muscle atrophy, whereas exercise is known to increase mitochondrial biogenesis and function. Here we provide evidence of short-term concurrent exercise training before an atrophic event protecting skeletal muscle from atrophy in two separate muscles with different, dominant fiber-types, and we reveal an association with the adaptive changes of mitochondrial molecular markers to exercise.

The present study aims to investigate if overexpressing the mitochondrial transcription factor A (TFAM) gene in a transgenic mouse model diminishes soleus and gastrocnemius atrophy occurring during hindlimb suspension (HLS). Additionally, we aim to observe if combining exercise training in TFAM transgenic mice prior to HLS has a synergistic effect in preventing skeletal muscle atrophy. Male C57BL/6J-based transgenic mice (12-14 weeks old) overexpressing TFAM were assigned to a control (T-Control), 7-day HLS (T-HLS), and 2-week exercise training prior to 7-day HLS (T-Ex+HLS) groups. These groups were compared to male C57BL/6J wildtype (WT) mice (12-14 weeks old) assigned to Control, 7-day HLS (HLS), 2week exercise training prior to 7-day HLS (Ex+HLS), and 2-week exercise training (Ex). Overexpressing TFAM results in a decrease of 8.3% in soleus and 2.6% in gastrocnemius muscle weight to bodyweight ratio after only HLS compared to wild-type mice incurring a loss of 27.1% in soleus and 21.5% in gastrocnemius muscle after HLS. Our data indicates TFAM may play a critical role in protecting skeletal muscle from disuse atrophy and is correlated with increased expression of antioxidants (SOD-2) and potential redox balance. TFAM may be an attractive molecule of interest for potential, future therapeutic development.

Human studies have shown high‐intensity interval training (HIIT) has beneficial cardiovascular effects and is typically more time‐efficient compared with traditional endurance exercise. The main goal of this study is to show the potential molecular and functional cardiovascular benefits of HIIT compared with endurance training (ET). Three groups of mice were used including sedentary‐control, ET mice, and HIIT mice groups. Results indicated ejection fraction was increased in HIIT compared with ET while fractional shortening was increased in the HIIT group compared with both groups. Blood flow of the abdominal aorta was increased in both exercise groups compared with control. Increases in cross‐sectional area and mitochondrial and antioxidative markers in HIIT compared with control were observed, along with several microRNAs. These findings indicate HIIT has specific cardiac‐protective effects and may be a viable alternative to traditional ET as a cardiovascular preventative medicine intervention.

Background: Human studies have shown high intensity interval training (HIIT) can have beneficial effects on the heart. The main goal of this study is to show 15 minutes of exercise with high intensity can has the same protective effects on the cardiovascular system as endurance training (ET) in healthy and diseased animals. Methods: For this study we used mouse (C57BL/6J) and rat (Wistar albino) models. We had three group of mice and rats: sedentary-control (CTRL), ET, and HIIT groups. First, 10-12 week old mice were treated with either ET or HIIT for 4 weeks. We performed heart ultrasonography, cardiomyocyte contractility, blood flow of the abdominal aorta and Western blot analysis for SOD-1, SOD-2, and citrate synthase to test cardiovascular differences between groups. Further, we used DHE staining, ATP production assay and O 2 - consumption kits. Second, 10-12 week old rats were treated with either ET of HIIT prior to ischemia/reperfusion injury on a Langendorff apparatus. Cardiodynamic data on dp/dt max, dp/dt min, SLVP, DLVP, heart rate, coronary flow and blood pressure were collected to observe differences between groups. Results: Our data reviled an increase in blood flow of mice abdominal aorta in both exercise groups compared to control but not between exercise groups itself (BF CTRL: 2218.7±10.0; BF ET: 2481.9±10.5; BF HIIT:2450.6±12.1). Ejection fraction was increased in HIIT compared to other groups (EF CTRL: 76.58±1.8; EF ET: 74.5±2.0 and EF HIIT: 84.83±2.4); Fractional shortening was increased in HIIT group compared to other groups (FS CTRL: 44.49±0.9; FS ET: 42.79±1.08; FS HIIT: 52.51±1.9). LIVDs was decreased in HIIT group compared to endurance, while in endurance was increased compare to control group (LIVDs CTRL: 1.11±0.3; LIVDs ET: 2.33±0.2; LIVDs HIIT: 1.4±0.2). DHE staining for ROS revealed no differences among groups. Conclusion: These findings indicate that HIIT have specific protective effects on the cardiovascular system compared to endurance training, even with possible higher heart contractility. People often cite the time as an excuse for lack of physical activity, however time is no longer an excuse.

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Thank you for being a source of positivity for me throughout this entire process. Spending time with each of you has been more helpful than you may know. To my sister and her husband, Krystal Atwater and Trey Atwater, I thank you for your consistent encouragement and care. Thank you to my brother, David Theilen, for being with me my entire life as a source of stability. Your work ethic, selflessness, and outstanding wife, Mary J. Theilen, have been positive influences on me that I am very thankful to have. To my mother, Kathy Nichols, for always supporting me. Your sacrifice led to my success, for which I will always be grateful. Finally, to my beautiful wife, Kathryn Marie Theilen: Thank you for always covering the details of life so I could spend more time on my development as a scientist. Your unwavering support throughout this entire process allowed me to thrive. You are the reason I want to succeed and I am incredibly thankful for you.