In order to expand the regional production of machine tools which utilize precision machined sliding guideways, cubic boron nitride (CBN) hard milling may be implemented as an alternative finish process instead of conventional surface grinding. While higher material removal rates can be achieved with a milling strategy, the use of a defined cutting edge leads to surfaces with asperities. These sharp peaks quickly wear down during sliding contact, resulting in unacceptable changes in the load bearing surface of the guide. Therefore, a post process to milling must be implemented to mitigate these features. This paper investigates the use of a spindle mounted abrasive disk filament tool for producing a functional surface similar to the ground surfaces. The optimal process parameters were investigated using 2D profile measurements, followed by comparison using areal analysis of ground, milled, and polished surfaces. It was found that polishing reduces both the height and volume of asperities on the milled surface, leading to contact characteristics which were more similar to those of the conventional ground surfaces currently used for sliding guideways.
The employment of the stable isotope labeling with amino acids in cell culture (SILAC) based proteomic analysis as a guidance tool for investigation of cellular response of a multifunctional nanodelivery system has been described for cancer therapy. The multifunctional nanodelivery system in this study was based on an estrogen anchored multimodal nanomicelle (NPG). The key components of the multifunctional nanomicelle consist: β-cyclodextrin conjugated estrone (CDE1), an escort molecule; polymeric BH3 mimetic, a proapoptotic BcL-2 inhibitor; and the mitotic catastrophe agent paclitaxel. They were assembled into the nanomicelle by multiple weak interactions including hydrophobic/hydrophilic interaction and host-guest recognition. The resulting nanomicelle exhibited unimodal morphology with average size of 170 nm with sustained release. It was found that the nanoparticle exhibits excellent anti-tumor activities for the treatment of breast adenocarcinoma with exceptional targeting efficacy both in MCF-7 tumor bearing mice. NPG as a new drug delivery system demonstrated several merits such as the increased drug uptake in breast tumor tissue, low toxicity, potent tumor growth retardation and metastasis inhibition, as well as potential clinical practicality without compromising liver, kidney and immune function and ameliorating the conventional chemotherapeutics induced phlebitis in breast tumor bearing nude mice model. The systematic SILAC based proteomics study and the subsequent validation revealed that the synergistic induction of mitotic catastrophe through enhanced G2/M phase arrest and PI3K/Akt/mTOR mediated autophagy, account for the exceedingly potent anti-tumor activity of this convergent nanomicelle. Additionally, the verification of the top upregulated gene from the proteomics profiling revealed that the overexpression of zinc finger protein 350 (ZNF350/ZBRK1) is associated with the enhanced antitumor effect induced by NPG.
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