Cross-fertilization and somatic embryogenesis stand as potential useful tools for genetic improvement and vulgarization of agronomical interesting cocoa planting material for sustainable cocoa culture. Manual cross-fertilization (♀SNK13×♂UPA143) was conducted. ♀SNK13×♂UPA143 derived hybrid genotypes were tested for their susceptibility to black pods disease (BPD). The most tolerant hybrid genotype (KHACa12) was monitored for its precocity, yielding and somatic embryo potential. Peroxidase and polyphenol oxidase activities were evaluated in association to embryogenesis in KHACa12. The cross-fertilization ♀SNK13×♂UPA143 generated around 60% of offprint's tolerant or less susceptible to BPD. When transferred in experimental farm, KHACa12 produced flowers 18 months after planting. Mature pods from KHACa12 bearded 55±5 seeds/pod. Seventy-five pods were harvested from KHACa12 during the first season. These pods generated 6 kg of fermented-dried cocoa seeds. Somatic embryo responsive revealed that 71.041 ± 7.91% and 50.64 ± 9.48% staminodes and petals derived explants, respectively produced direct and indirect somatic embryos. Morphologically, the somatic embryos obtained were similar to zygotic embryos from the same hybrid genotype (KHACa12). Peroxidase and polyphenol oxidase activities profiles in zygotic and somatic embryos increase during the first stages of embryogenesis, then decrease when cotyledons developed. These results might indicate that, ♀SNK13×♂UPA143 could be used to develop cocoa (Theobroma cacao L.) hybrid genotypes tolerant or less susceptible to BPD. KHACa12 is an agronomic interesting hybrid genotype that could be produced in large scale and vulgarized using somatic embryogenesis process. The initiation and development of somatic embryo in KHACa12 can be monitored and modulated biochemically through peroxidase and polyphenol oxidase activities profiles.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.