<p><em>Plant extracts as a botanical insecticide can be used in either single or mixture forms. Mixed extracts have advantages: reducing dependence on one plant species, being the synergistic effect which can increase the effectiveness of formulas, and reducing the possibility of resistance emergence. This study aimed to obtain compatible and synergistic comparisons of </em>P. retrofractum<em> with </em>C. xanthorrhiza<em> extracts that effectively control the third instar nymph of </em>Helopeltis antonii<em>. The comparison of </em>P. retrofractum<em> and </em>C. xanthorrhiza<em> extracts were 4:1, 2:1, 1:1, 1:2, and 1:4 (w/w). The experiment was performed in a completely randomized design using the contact method. The LC<sub>50</sub>, LC<sub>90</sub>, and LC<sub>95</sub> values were calculated using the PoloPlus Ver 1.0 Program. The mixture interaction was determined from the Combination Index value. The results showed that</em> P. retrofractum<em> extract was compatible and synergistic with </em>C. xanthorrhiza<em> extract at a ratio of 2:1 (w/w). These were the best combination shown by the lowest LC<sub>50</sub>, LC<sub>90</sub>, and LC<sub>95</sub> values and showed consistently strong synergistic properties compared to other concentrations. The components of</em><em> </em><em>P. retrofractum</em><em> </em><em>may be responsible for the insecticidal properties are piperine; piperanine; piperidine; 3,4-Pyridinedimethanol,6-methyl; Piperidine,1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-,(Z,Z)-; and (2E,4E,12E)-1-(Piperidine-1-yl)octadeca-2,4,12—trien-1-one. The insecticidal activity of </em>C. xanthorrhiza<em> may associated with its content of</em><em> xanthorrhizol, Ar-curcumene, β-curcumene, curzerene, germacrone</em>, <em>7-zingiberene, caryophyllene, and β-bisabolol. A combination of these two plant extracts has the potential to be further developed as a botanical insecticide to control</em> H. antonii<em>.</em></p>
Tribolium castaneum merupakan salah satu hama gudang penting di negara tropis. Fumigasi menggunakan fosfin adalah upaya pengendalian yang paling umum dilakukan. Namun, penggunaan fosfin secara terus-menerus dalam waktu lama telah mengakibatkan terjadinya resistensi. Tujuan penelitian ini untuk mempelajari efek fumigan dan repelen fraksi-fraksi minyak pepermin Mentha piperita terhadap T. castaneum, serta mengidentifikasi senyawa-senyawa yang terkandung di dalam fraksi aktifnya. Penelitian dilakukan di Laboratorium Entomologi Seameo Biotrop Bogor dan di Pusat Laboratorium Forensik Mabes Polri Jakarta sejak Januari hingga Oktober 2016. Minyak pepermin didestilasi dari daun M. piperita. Minyak pepermin difraksinasi dengan teknik partisi pelarut menggunakan pelarut n-heksana, etil asetat, dan metanol. Ketiga fraksi diuji sebagai fumigan dan repelen terhadap T. castaneum. Fraksi aktif dianalisis senyawa kimianya menggunakan GC-MS. Fraksi n-heksana pepermin (FHP) merupakan fraksi yang aktif terhadap mortalitas imago dan larva T. castaneum. Nilai LC 95 FHP untuk imago dan larva berturut-turut sebesar 1,92 % dan 9,43 %. Konsentrasi sublethal FHP juga menunjukkan aktivitas menghambat perkembangan larva. Persentase larva berhasil menjadi pupa berkisar 48,67-74,91 %, sedangkan yang berhasil menjadi imago berkisar 9,12-16,59 %. FHP memiliki aktifitas repelen terhadap imago T. castaneum mencapai 86,7 % pada 72 jam setelah perlakuan (JSP). Senyawa dominan pada FHP antara lain β-pinen, limonen, isopulegol, menton, isomenton, mentol, pulegon, dan trans-carane. FHP atau senyawa-senyawa yang dikandungnya berpotensi untuk dikembangkan sebagai insektisida nabati.
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