In this study, an Inverse Phase‐High Performance Liquid Chromatography method was developed for the determination of the quantities of Etodolac and Thiocolchicoside active ingredients contained in the same tablet form (Etotio tablet). For this purpose; Etodolac and Thiocolchicoside in the pharmaceutical combination were determined simultaneously using Inverse Phase‐High Performance Liquid Chromatography method. This developed high‐performance liquid chromatography method was also used for the simultaneous in vitro quantity determination of Etodolac and Thiocolchicoside in human plasma. Plasma samples were analyzed with the Inverse Phase‐High Performance Liquid Chromatography method without any need for extraction process and very high recoveries were obtained with this chromatographic method. Method validity was tested by applying all the validation parameters for the data obtained from both studies (in solvent and plasma media). Results obtained in plasma media and solvent media were compared statistically with Anova test and it was found that there is no significant difference (at 95% possibility level) between the two methods from average and standard deviation aspects.
DOI: http://doi.org/10.1002/sscp.201900036
The cover picture shows an easy, fast, economical, repeatable, sensitive and new Reverse Phase‐High Performance Liquid Chromatography method been developed for the simultaneous quantitative estimation of Etodolac and Thiocolchicoside combination in human plasma (in‐vitro). Three‐dimensional spectrum of Thiocolchicoside and Etodolac in plasma. Determination of Etodolac and Thiocolchicoside without any need for extraction process in human plasma was carried out at 259 nm by using Photo Diode Array Detector as seen from the three‐dimensional spectrum of Thiocolchicoside and Etodolac in plasma. Very high recovery values as 97.50–100.24% for Etodolac and 97.21–100.68% for Thiocolchicoside were obtained. Furthermore, an environmental friendly and more economical method was developed by decreasing the methanol rate within the mobile phase mixture. This method was also applied in human plasma media and it was seen that it can be easily and successfully applied in routine quantity analysis processes.
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