MPI‐CDG is a rare congenital disorder of glycosylation (CDG) which presents with hepato‐gastrointestinal symptoms and hypoglycemia. We report on hepatic evaluation of two pediatric patients who presented to us with gastrointestinal symptoms. Analysis of carbohydrate deficient transferrin (CDT) showed a Type 1 pattern and molecular analysis confirmed the diagnosis of MPI‐CDG. Oral mannose therapy was markedly effective in one patient but was only partially effective in the other who showed progressive portal hypertension.
Objectives: This study was aimed to analyzing the phenolic constituents of the 80% methanol extracts of three Abutilon species (A. pannosum, A. hybridum and A. hirtum) (family Malvaceae). Methods: Defatted 80% methanol extracts of A. pannosum, A. hybridum and A. hirtum aerial parts (leaves and stems) were, each, analyzed using high performance liquid chromatographic (HPLC) technique against external standard calibration at λ 280 and 330 nm. Results: Twelve phenolic acids were tentatively identified from A. pannosum, A. hybridum and A. hirtum representing 9.03, 12.23 and 20.51%, respectively. The major identified phenolic acids peaks were that of protocatechuic acid in A. pannosum (1.75%), ferulic acid in A. hybridum (2.09%) and ellagic acid in A. hirtum (4.0%). On the other hand, 21 flavonoids were tentatively identified from A. pannosum representing (53.78%), in comparison with 20 identified from A. hybridum (42.12%) and 19 identified from A. hirtum (35.68%). Kaempferol 3-O-[2 \\ -p-coumaroyl-β-D-glucopyranoside] was the major identified flavonoid glycoside in A. pannosum and A. hybridum methanolic extracts (13.27 and 5.13%, respectively) while quercitrin was the major flavonoid glycoside identified in A. hirtum (3.21%). The major flavonoid aglycones identified were luteolin (3.68%) in A. pannosum, apigenin (9.46%) in A. hybridum and quercetin (5.85%) in A. hirtum. Conclusion: The present study revealed that the investigated extracts are rich in phenolic contents; A. pannosum has the highest concentration of flavonoids and the least concentration of phenolic acids, where A. hirtum has the highest concentration of phenolic acids, showing promising phenolic content for further study of their isolation, identification as well as evaluation of their biological activity.
Objectives: In the present study, a comparative evaluation of the lipoidal matter of the aerial parts (leaves and stems) of three Abutilon (A.) species, A. pannosum (Forst.f) Schltdl., A. hybridum Hort. (Ex Siebert. & Voss), and A. hirtum (Lam.) Sweet (family Malvaceae) has been carried out for the first time. Methods: Gas-liquid chromatography coupled with flame ionization detector (GLC/FID) was used for analyzing and identifying the saponifiable and unsaponifiable compounds. Results: Saponification of lipoidal matter of the petroleum ether extracts of A. pannosum, A. hybridum, and A. hirtum revealed the presence of 23 compounds tentatively identified in the unsaponifiable matter of A. pannosum, 19 in A. hybridum, and 22 compounds in A. hirtum. Whereas n-heneicosane, β-sitosterol, n-tricosane were the most abundant compounds identified in the unsaponifiable matter of the aerial parts of A. pannosum, A. hybridum and A. hirtum, respectively. In the saponifiable fractions, a total of 14 compounds were specified for A. pannosum, 18 for A. hybridum and 19 compounds for A. hirtum. Palmitic acid was the major identified saturated fatty acid in the three Abutilon species accounting for 23.18% for A. pannosum, 23.97% for A. hybridum, and 19.23% for A. hirtum, while oleic acid represents the major unsaturated fatty acid in the three species (17.78, 34.44 and 22.13%, respectively). Conclusion: Lipoidal matter investigation by Gas-liquid chromatography coupled with flame ionization detector (GLC/FID) was used for the tentative identification of different bioactive lipoidal constituents as hydrocarbons, sterols, unsaturated and saturated fatty acids from A. pannosum, A. hybridum, and A. hirtum. The results showed that the three Abutilon species are worthy candidates for further pharmacological and phytochemical studies. The present study is the first report on the petroleum ether extracts of A. hybridum, A. hirtum plants and the first report on the aerial parts of A. pannosum.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.