Growing concerns over ecosystem degradation, climate change, loss in biodiversity, and rapid depletion of natural resources have urged societies of the developed countries all over the world to encounter the challenge of shifting from fossil-based to bio-based economies. With European Green Deal priorities on a transition to a climate-neutral economy with net-zero GHGs emissions by 2050, projected demand for biomass is 40–100% higher, relative to its supply. To provide an overview on the capacity of the European Union countries to satisfy its demand for biomass through the organic materials extracted directly from the domestic natural environment, the study aimed to assess the biomass self-sufficiency based on its domestic extraction-domestic consumption balance. Both the spatial and temporal variability of the self-sufficiency ratio are used to characterise the stability of capacity to satisfy our own needs for biomass in the EU economy as a whole, and at the level of individual member states. The findings indicate that the differences in biomass self-sufficiency ratios are quite high among the European Union member countries (i.e., in the range of 15% in Malta and 33% in Cyprus; up to 184% in Estonia and 224% in Latvia (on average in 2016–2018)). GMM analysis (EU-28, 2000–2018 period) is provided in this study to define the main statistically significant factors that have an impact on the biomass self-sufficiency ratio. This study contributes to the debate on the issues of biomass self-sufficiency in the context of ecological constraint and the EU’s Green Deal.

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