While several studies have investigated mouse ultrasonic vocalizations (USVs) emitted by isolated pups or by males in mating contexts, studies of behavioral contexts other than mating and vocalization categories other than USVs have been limited. By improving our understanding of the vocalizations emitted by mice across behavioral contexts, we will better understand the natural vocal behavior of mice and better interpret vocalizations from mouse models of disease. Hypothesizing that mouse vocal behavior would differ depending on behavioral context, we recorded vocalizations from male CBA/CaJ mice across three behavioral contexts including mating, isolation, and restraint. We found that brief restraint elevated blood corticosterone levels of mice, indicating increased stress relative to isolation. Further, after 3 days of brief restraint, mice displayed behavioral changes indicative of stress. These persisted for at least 2 days after restraint. Contextual differences in mouse vocal behavior were striking and robust across animals. Thus, while USVs were the most common vocalization type across contexts, the spectrotemporal features of USVs were context-dependent. Compared to the mating context, vocalizations during isolation and restraint displayed a broader frequency range, with a greater emphasis on frequencies below 50 kHz. These contexts also included more non-USV vocal categories and different vocal patterns. We identified a new Mid-Frequency Vocalization, a tonal vocalization with fundamental frequencies below 18 kHz, which was almost exclusively emitted by mice undergoing restraint stress. These differences combine to form vocal behavior that is grossly different among behavioral contexts and may reflect the level of anxiety in these contexts.

Background: Electronic cigarettes (e-cigarettes) are often thought to be a healthier option to cigarette smoking. e-Cigarettes have been found to overheat and explode. e-Cigarette explosions have caused severe trauma and rendered patients in critical conditions. Inadvertent exposures to liquid nicotine products have caused systemic poisoning injuries. We sought to characterize e-cigarette injuries presenting to emergency departments (ED) in 2018.Methods: We analyzed one year of data from the US Consumer Product Safety Commission’s National Electronic Injury Surveillance System (NEISS). Patients presenting with injuries associated with e-cigarette products were manually identified for inclusion. We performed descriptive analyses on demographic factors, affected bodily regions, dispositions, locations of occurrence, and mechanisms of injury. By applying sample weights, nationally representative estimates were calculated.Results: A total of 361 667 injury cases were reported in NEISS (2018). We identified 50 e-cigarette injury cases, generating a national estimate of 1739 (95% CI [1333-2148]) patients presenting to US EDs with e-cigarette injuries in 2018. Approximately 1000 pediatric patients (age ≤17 years) and 700 adult patients (age ≥18 years) were included. The median age when presenting to the ED was 4 years (interquartile range [IQR], 1-25). Over 85% of injuries occurred at home. Ingestion (55.0%) was the most common mechanism of injury, followed by explosion (35.8%).Conclusion: Children and adults are susceptible to injury from e-cigarette products. Changes in manufacturing standards may prevent injuries from these products.

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Background: Adverse childhood experiences (ACEs) are potentially traumatic incidents occurring before age 18 years. Adverse childhood experiences include physical or mental abuse, financial stress, home or community violence, substance misuse, familial turmoil, and other factors. Adverse childhood experiences are associated with negative health outcomes in adulthood. Methods: Numerous research studies and systematic reviews were reviewed to assess the breadth and depth of racial and ethnic inclusivity in ACE research. Results: A wide range of ACEs have been investigated, and ample state-level data is publicly available. Early, fundamental ACE studies typically recruited White, educated, and insured participants; racial and ethnic diversity were often neglected. Conclusion: Adverse childhood experiences and race have been found exerting synergistic effects on adult health outcomes. Further evaluation of race is warranted to improve health outcomes. Scrutiny of racial and ethnic equity in health research is paramount for achieving health equity.