The influence of laundry washing parameters on the release of microfibers (MF) from polyester textiles was studied. These fibers are an important type of microplastic pollution. However, the factors which affect MF release during laundry are poorly understood and more rigorous methods for quantifying this release are needed. A novel method was therefore developed using a tergotometer with eight 1000 mL washing vessels and the CIELab color space measure of lightness (L*). L* was related to the mass of released MFs by creating a calibration curve to quantify the amounts of MFs released from textiles during washing. This method was used to investigate the effect of water-volume, agitation, temperature, and duration of the wash on MF release. Counterintuitively, increased water-volume, characteristic of European "delicate" cycles, resulted in the greatest release of MFs. Full-scale testing was then carried out using domestic washing machines with real consumer cycles to determine the effect of cycle type on MF release. In the first wash, delicate wash cycles released 800 000 more MFs (94 mg/kg) per wash than a lower water-volume standard wash and also increased MF release in subsequent washing cycles (P < 0.05). These results indicate that a high water-volume-to-fabric ratio is the most influential factor for MF release, rather than agitation as previously thought. Therefore, consumers can reduce MF release by avoiding high water-volume washes (delicate cycles), transitioning to appliances that use a lower water-volume (North American high-efficiency washing machines), and ensuring that full wash loads are used.

Fiber release during domestic textile washing is a cause of marine microplastic pollution, but better understanding of the magnitude of the issue and role of fabric care products, appliances and washing cycles is needed. Soiled consumer wash loads from U.K. households were found to release a mean of 114 ± 66.8 ppm (mg microfiber per kg fabric) (n = 79) fibers during typical washing conditions and these were mainly composed of natural fibers. Microfiber release decreased with increasing wash load size and hence decreasing water to fabric ratio, with mean microfiber release from wash loads in the mass range 1.0-3.5 kg (n = 57) found to be 132.4 ± 68.6 ppm, significantly (p = 3.3 x 10 −8) higher than the 66.3 ± 27.0 ppm of those in the 3.5-6.0 kg range (n = 22). In further tests with similar soiled consumer wash loads, moving to colder and quicker washing cycles (i.e. 15˚C for 30 mins, as opposed to 40˚C for 85 mins) significantly reduced microfiber generation by 30% (p = 0.036) and reduced whiteness loss by 42% (p = 0.000) through reduced dye transfer and soil re-deposition, compared to conventional 40˚C cycles. In multicycle technical testing, detergent pods were selected for investigation and found to have no impact on microfiber release compared to washing in water alone. Fabric softeners were also found to have no direct impact on microfiber release in testing under both European and North American washing conditions. Extended testing of polyester fleece garments up to a 48-wash cycle history under European conditions found that microfiber release significantly reduced to a consistent low level of 28.7 ± 10.9 ppm from eight through 64 washes. Emerging North American High-Efficiency top-loading washing machines generated significantly lower microfiber release than traditional top-loading machines, likely due to their lower water fill volumes and hence lower water to fabric ratio, with a 69.7% reduction observed for polyester fleece (n = 32, p = 7.9 x 10 −6) and 37.4% reduction for polyester T-shirt (n = 32, p = 0.0032). These results conclude that consumers can directly reduce the levels of microfibers generated per wash during domestic textile washing by using colder and quicker wash cycles, washing complete (but not overfilled) loads, and (in North America) converting to High-Efficiency washing machines. Moving to colder and quicker cycles will also indirectly reduce microfiber release by extending the lifetime of clothing, leading to fewer new garments being purchased and

Cellobiose dehydrogenase from the ascomycete fungus Myriococcum thermophilum (MtCDH) was tested for the ability to generate bleaching species at a pH suitable for liquid detergents. The catalytic properties of MtCDH were investigated for a large variety of carbohydrate substrates using oxygen as an electron receptor. MtCDH produces H2O2 with all substrates tested (except fructose) but only in the presence of a chelant. Insoluble substrates like cellulose and cotton could as well be oxidized by MtCDH. To enhance the amount of cello-oligosaccharides in solution, different cellulases on cotton were used and in combination with MtCDH an increased H2O2 concentration could be measured. Additionally, the degradation of pure anthocyanins in solution (as model substrates for bleaching) was investigated in the absence and presence of a horseradish peroxidase. MtCDH was able to produce a sufficient amount of H2O2 to decolorize the anthocyanins within 2 h.

This is a repository copy of Improved garment longevity and reduced microfibre release are important sustainability benefits of laundering in colder and quicker washing machine cycles.

The carbohydrate oxidase from Microdochium nivale (CAOX), heterologously expressed in Aspergillus oryzae, and cellobiose dehydrogenase from Myriococcum thermophilum (MtCDH), were assessed for their ability to generate bleaching species at a pH suitable for liquid detergents. The substrate specificities of CAOX and MtCDH were analyzed on a large variety of soluble and insoluble substrates, using oxygen as an electron receptor. Even insoluble substrates like cellulose were oxidized from both CAOX and MtCDH, but only MtCDH produced H₂O₂ on cotton as the sole substrate. To enhance the amount of cello-oligosaccharides formed from cotton as substrates for CAOX and MtCDH, various cellulases were used in combination with MtCDH or CAOX, leading to a 10-fold increase in H₂O₂. As model substrates for colored stains, the degradation of pure anthocyanins and stain removal of blueberry stains by CAOX and MtCDH was examined in the absence and presence of a horseradish peroxidase. Both enzymes were able to produce an amount of H₂O₂ sufficient to decolorize the pure anthocyanins within 2 h and showed significant cleaning benefits on the stains.

Vented tumble dryers release moist warm air from the drying process to the external environment, usually through pipework linking the appliance to a vent in an exterior wall. Although such dryers contain a lint filter to remove fibers from this air stream, recent reports suggest that this process is incomplete, leading to microfibers being released in the ducted warm air and subsequently polluting the external environment. Microfiber release from wash loads comprising 10 100% cotton and 10 100% polyester T-shirts (total load mass ratio 48% cotton, 52% polyester) was measured at different stages of the washing and drying process to compare the quantities of fibers released ‘down the drain’, collected in the dryer lint filter, and released to air from the tumble dryer. Testing under both European and North American washing conditions found that the quantities of microfibers released to air during tumble drying were significant and comparable to levels released ‘down the drain’ during washing. Use of conventional rinse-added liquid fabric conditioner increased microfiber accumulation on the dryer lint filter, with reduced release from the dryer exhaust observed at the highest fabric conditioner dose tested (21.6% and 14.2% reduction under North American and European conditions, respectively). Conventional liquid fabric conditioner did not significantly impact microfiber release from the washing machine, in line with previous studies. A fabric conditioner specially designed for anti-wrinkle performance reduced microfiber release from the dryer exhaust at all levels tested (by 17.6–35.6%, depending on dose), apparently by increasing the efficiency of microfiber accumulation in the lint filter. Tumble dryer sheets were also found to cause a reduction in microfiber release from the dryer exhaust (by 14.1–34.9%, depending on the dose/product), likely driven by collection of liberated fibers on the sheet during the drying process. The use of both antiwrinkle liquid fabric conditioner and dryer sheet enabled a 44.9% reduction in microfiber emissions from the dryer exhaust. In all studies, the fiber mass collected on the lint filter or emitted from the dryer exhaust was richer in cotton fibers (range 83.4–96.3% on the lint filter, 93.0–99.8% from the dryer exhaust) than the wash load composition (48% cotton). Moreover, fibers collected by the lint filter contained a higher proportion of polyester than emissions from the dryer exhaust (range 3.7–16.6% on the lint filter, 0.2–7.0% from the dryer exhaust). There is significant variation in the porosity of lint filters among installed vented tumble dryers. Single-variable testing of the impact of lint filter design concluded that reducing screen pore size significantly reduces airborne microfiber release during tumble drying; a reduction in lint filter pore size from 0.2 mm2 to 0.04 mm2 reduced release by 34.8%. As some lint filters have pore sizes of around 1 mm2, there is enormous scope to reduce microfiber release from dryers though improved lint filter design. However, it is suggested that a step-change in appliance design away from vented dryers to only fully-sealed condenser dryers might be necessary to eliminate the contribution of tumble drying to airborne microfiber pollution.

Force spectroscopy was used to show that extracellular DNA (eDNA) has the pre-eminent structural role in a biofilm. The adhesive behavior of extracellular polymeric substances to poly(ethylene terephthalate), a model hydrophobic surface, was measured in response to their degradation by hydrolytic enzymes known for their biofilm-dispersion potential: DNaseI, protease, cellulase, and mannanase. Only treatment with DNaseI significantly decreased the adhesive force of the model bacterium Micrococcus luteus with the surface, and furthermore this treatment almost completely eliminated any components of the biofilm maintaining the adhesion, establishing a key structural role for eDNA. ASSOCIATED CONTENT Supporting Information. Contact angle methods, results of biofilm coated silicon wafer after enzyme treatments, fluorescence image of biofilm-coated cantilever, and forcedistance control curves showing poly-lysine contacting a PET substrate. This material is available free of charge via the Internet at http://pubs.acs.org.

The inclusion of a nuclease, a new‐to‐laundry enzyme class in detergent formulations, reduces the concentration of recalcitrant soils on dirty clothing. Nucleases target extracellular DNA that, albeit present in minor quantities in soiled laundry items, disproportionately contributes to the accumulation of sebaceous soils and other compounds that affect the appearance of clothes. These lingering soils accumulate over multiple wear cycles and provoke a dingy appearance in clothing, characterized by dull colors, yellow staining, and an unpleasant malodor. The single and continued use of a nuclease in detergent formulations offers increased removal of these recalcitrant soils to produce less malodorous and visually preferred textiles. Furthermore, the nuclease is effective across different detergent forms, as well as in cotton short washing cycles, enabling improved cleaning in more sustainable laundry washing conditions.