Arezou Taghvimi contributed equally to this work.Determination of methamphetamine is of great importance in clinical and forensic laboratories because there are low dosages of drugs in the biological media and social problems created due to the methamphetamine consumption. Polymeric carbon based-nano composites are reasonable candidates for dispersive solid phase extraction method due to facial and affordable synthesis process and high selectivity and sensitivity. Nano graphene oxide polypyrolle composite was synthesized and employed as dispersive solid-phase extraction adsorbent for methamphetamine extraction from complex urine matrix. Full characterization of the prepared nano graphene oxide polypyrolle composite was completed and the influential extraction parameters were investigated through one-parameter-at-a-time method. High-performance liquid chromatography detectors were applied as detection and quantification instrument. The optimized extraction parameters included 300 μL of methanol, 10 min of extraction and desorption time, 6000 stirring rate, urine pH value of 10, 60 mg of adsorbent, and 6 mL of urine volume. After outlining the calibration curve, the linear range of the method was considered as 30-800 ng/mL. The detection limit for the suggested method was 9 ng/mL. The analysis of addicted subjects with the proposed method confirmed the utility of the method in different analytical and clinical laboratories. K E Y W O R D Sdispersive solid-phase extraction, graphene oxide, methamphetamine, polypyrrole, urine 606 How to cite this article: Jabbari NR, Taghvimi A, Dastmalchi S, Javadzadeh Y. Dispersive solid-phase extraction adsorbent of methamphetamine using insitu synthesized carbon-based conductive polypyrrole nanocomposite: focus on clinical applications in human urine. J Sep Sci 2020;43:606-613. https://doi.