Antifungal activity of Thymus daenensis and Satureja hortensis essential oils (EOs) (0, 50, 100, and 150 ppm) were evaluated against Aspergillus fumigatus in vitro and in the Food model system (un‐heat and heat‐treated Doogh) during 21 days. In vitro results showed that minimum inhibitory concentration and minimum fungicidal concentration of T. daenensis and S. hortensis oils were 6.25 and 12.5%, respectively. In un‐heat treated samples, both EOs showed a significant inhibitory effects rather than the control group at 25 °C than 4 °C. Treatments containing 100 and 150 ppm of T. daenensis EO on the 21st day and treatment containing 50 ppm of S. hortensis EO on the 15th day had maximum inhibitory effect. No growth was observed in the heat‐treated samples and control group. The findings revealed that both EOs had noticeable antifungal activity and can be considered as a natural preservative in the food industry. Practical applications Doogh is a traditional Iranian dairy‐based drink which is the same as other dairy fermented products prone to fungal contamination. As a result, application of chemical and synthetic preservatives is common to control microbial growth and increase the shelf life. But, due to their harmful side effects, much research has been being conducted to find a suitable substitute like plant EOs, as natural preservatives in the food industry. This study assessed the antifungal activity of T. daenensis and S. hortensis EOs and their potential applications in food model system.
Doogh is an Iranian traditional dairy-based drink product formally identified as Iranian national drink. This fermented acidified drink has high nutritional value and pleasant organoleptic properties. Samples (n= 120) collected from both industrial and traditional Doogh retail stores in Hamadan in 2015 were analyzed for total solids (%), pH, titratable acidity, salt (%), mold and yeasts count (cfu/ml) according to the Iranian National Standards (no. 2453). Results showed that 100% and 24% of the traditional and industrial Doogh samples had fungal contamination over than 100 cfu/ml, respectively. Moreover, the mean values of titratable acidity, pH, salt, and total solids showed a significant between-group difference. According to this study, employing hygiene programs regarding the production processes is highly recommended.
The combined effects of lipase/cream reaction, temperature/time and pH of fermentation on levels of free fatty acids (FFAs), biochemical changes, oxidative stability and flavour development of the dough during 21 days of cold storage were investigated. Levels of short-chain FFAs increased in doogh samples, while long-chain FFAs decreased. Under the severe conditions of the lipase/cream reaction, the lowest pH drop, acidity increase and the highest intensity of lipid oxidation were observed. Treatment with a shorter reaction time and a lower temperature of the lipase/cream mixture and a lower final pH of fermentation (4.0-20-30) achieved the highest sensory acceptability, which could be used to improve industrial doogh.
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