The study was conducted with the aim to assess the knowledge of segregation of needles and syringes among staff nurses in Child Health Care Areas of Christian Medical College (CMC) and Hospital, Ludhiana, Punjab. The objectives of the study were to assess the knowledge, find out the correlation between knowledge with selected variables and identify the deficit in areas of knowledge. Non-experimental research design and descriptive approach was used. Purposive sampling technique was used and sample size was 60. Self-Structured knowledge questionnaire was used to assess the knowledge. Data analysis was done using descriptive and inferential statistics. Findings revealed that majority of the staff nurses had average knowledge with maximum in the area of complications of inappropriate segregation and minimum regarding legal aspects. Selected variables such as age, work experience, professional qualification, in-service education, marital status and training institute had no impact on knowledge. Maximum knowledge deficit was regarding the authorities which make guidelines and decide penalty and least regarding primary source and color coding of biomedical waste. Lesson plan was devised to increase the knowledge of staff nurses.
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