Determination of the effect of Cotton wilt disease (Verticillium dahliae Kleb.) on seed contentThe study was carried out to determine the effect of Cotton wilt disease (Verticillium dahliae) on seed content, the experiment set up in randomized blocks design methods with four replications in Dicle University, Agricultural Faculty Research Area that is naturally infested with the pathogen in Diyarbakır in 2014. Stoneville 468 (Moderately tolerant) and Beyaz Altın 119 (Moderately susceptible) cotton varieties are used in the study. The land prepared for sowing and cotton seeds were sown in the 23th of May, 2014. Necessary agricultural practices were performed until the harvest. During the harvest, the stem of plants were cut 5-6 cm above ground level for each plot and the disease severity was recorded according to 0-3 disease scales. Also, in Diyarbakır and Batman, 5 farmers field were checked in the same way and the plants were evaluated according to the 0-3 scale respectively. During the harvest 40-50 g cotton seeds were brought to the laboratory for each character. After ginning, cotton seeds were treated with sulfuric acid to delint at the laboratory. After that delinted seeds were grinded with a mixer for seed content analysis. The prepared samples were stored at -18⁰C in a deep freeze. The samples were kept in a pastor oven at 70 0 C for 24 hours for their humidity to stabilize. Then the seed contents were analyzed by "Nor-XDS Rapid Content Analyser" device. According to 0-3 disease scale, protein content, oil content, ash content, starch content and cellulose content of seeds were found to be significantly different at the farmers' fields and research area and moisture content were not determined differently at the farmers field, but were found differently in research area.
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