Aim: To observe the changes in the caliber of hepatic central vein of Albino rats exposed to the oral administration of toxic doses of Imatinib and to assess the protective effect of vitamin E. Study design: Experimental study Place and duration of study: Department of Pharmacy, Peshawar Medical College Peshawar from 1st October 2015 to 31st March 2016. Methodology: Twenty four male Sprague-Dawley rats (150- 200g) were selected for this study and these rats were divided into three groups. Group 1 was control group, Group II received oral Imatinib solution (50 mg/kg/day) and Group III received Imatinib solution (50mg/kg/day) orally along with vitamin- E (500MG/kg/day) for 14 days. At the end of experiment central vein dilatation in liver tissue of rats were observed. Results: The increase in mean central vein dilatation in liver of group II were statistically significant (p=0.001) in comparison with the control. The mean central vein caliber of liver was decreased significantly in group III. Conclusion: The simultaneous use of vitamin E as an antioxidant with Imatinib can protect the toxic effects of the Imatinib on hepatic toxicity which includes central vein dilatation. Keywords: Imatinib mesylate, Hepatic tissue, Central vein dilatation, Vitamin E
Objective: To evaluate histomorphological effects of Soybean oil supplementation on lung tissue injury and alveolar hemorrhage induced by Bisphenol A (BPA). Study Design: Laboratory-based experimental study. Place and Duration of Study: Anatomy department, Army Medical College, Rawalpindi and National Institute of Health, Islamabad, from Nov 2015 to Nov 2016. Methodology: Forty (40) healthy BALB/c mice of 9-11 weeks of age, weighing between 30-37gm were housed in a controlled environment at National Institute of Health. Group 1 (10) was control group. Group 2 (10) was given a daily dose of 50 milligram/kilogram body weight of Bisphenol A and group 3 (10) was given a daily dose of 500 milligram of Soybean oil and group 4 (10) was concurrently given Bisphenol A and Soybean oil with daily doses of 50 milligram/kilogram body weight and 500 milligrams. After a period of 8 weeks, animals were dissected 24 hours after receiving the last dosage. Lung wet weight, animal weight and relative body tissue weight index (RTBWI) were calculated. Tissue processing & staining was done. Alveolar hemorrhage was histomorphologically and statistically analysed using SPSS-21. Results: On microscopic examination, alveolar hemorrhage (AH) was observed in 10 (100%) group 2 specimens with increase in RTBWI and whereas only 5 (50%) of group 4 specimens had alveolar hemorrhage with slight improvement in relative body tissue weight index (RTBWI). Conclusion: Bisphenol A (BPA) induced lung injury as evident by intraalveolar hemorrhage, blood vessel congestion and increased RTBWI ratio were ameliorated by concomitant administration of Soybean oil.
Background. Drugs induced liver injury is a major health problem.1 Methotrexate which is antagonist of folic acid is used to treat different types of neoplasms, rheumatoid arthritis and psoriasis.2 The therapeutic applications of Methotrexate is usually limited by its severe Hepatotoxicity. Herbs play important role for the treatment of various liver diseases.3 Turmeric (Curcuma Longa L, zingiberaceae) is used to treat cancer, inflammatory disorders, hepatitis and other liver disorders, skin diseases, Alzheimer’s disease, rheumatoid arthritis.4 Study Design: Laboratory based randomized controlled trial. Setting: Animal House of IBMS (Institute of Basic Medical Science), by the permission of Ethical Committee of (KMU) Khyber Medical University Hayatabad Peshawar. Period: March to September 2018. Material & Methods. 28 adult male albino mice were divided into control Group A and experimental Groups B,C and D. Group B was given Turmeric extract per oral (400mg /kg) daily for 14 days. Group C was given Injection Methotrexate (40mg/kg) intraperitoneally (I.P) on day 7. Group D was given Turmeric extract per oral (400mg /kg) daily for 7 days as pre administration to injection Methotrexate and on day 7 injection Methotrexate (40mg/kg) was given intraperitoneally (I.P) and Turmeric extract per oral (400mg/kg) was given daily for further 7 days as post administration to injection Methotrexate. Histological slides were prepared to see the effects of Turmeric plus Methotrexate on diameter of central vein and congestion in central vein. Results: The histological examination of liver sections of control group. A showed normal appearance of central vein. In Turmeric treated group (Group B) also no obvious histological changes were observed. Examination of liver tissue of Group C (Methotrexate group) showed severe histological changes which include dilation and congestion of central vein. Group D (Methotrexate + Turmeric group) showed significant reduction in dilation and congestion of central vein. Conclusion: This study is in accordance with other studies in which different types of herbals showed protective effects on Methotrexate induced liver damage.
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