The present study intended to examine the impact of methodology on EFL vocabulary learning of elementary school boys and girls. To achieve this end, 40 elementary female and male students aged 9-10 were selected from among 60 students studying at a language institute in Isfahan, Iran. The students were selected based on the results of an overall language proficiency test as a placement test that had been prepared by the institute that identified students' level of proficiency. They were further divided into two experimental groups. Next, a pretest was used to identify the number of words students knew before treatment. The experimental group A learned vocabulary through Direct Method and in the experimental group B students learned vocabulary through Total Physical Response. After 12 weeks of instruction a post-test was administrated to measure and compare the results of vocabulary learning of two groups after treatment. The data collected were put to statistical analysis using SPSS. The results of t-test showed the positive effect of TPR on learners' L2 vocabulary knowledge. With regard to the impact of gender on learners' L2 vocabulary knowledge, findings revealed that there were not any statistically significant differences between the male and female learners' vocabulary score.