Background: In terms of diagnosis and management of diabetes mellitus, the most commonly requested core biochemistry test worldwide is measurement specimen preservative, its use has been critically questioned. Objective: K 2 C 2 O 4) tubes in a routine laboratory setup. Methods: Sixty one pairs of blood specimens were collected into plain tubes and NaF/K 2 C 2 O 4 tubes. Following separation of plasma (NaF/K 2 C 2 O 4 tubes) and serum (plain tubes), glucose concentration was measured. Five determinants were obtained; the baseline glucose value, glucose concentrations at one, two, four and six hours after collection of blood into NaF/K 2 C 2 O 4 or plain tubes. Results: A high rate of haemolysis was observed in NaF/K 2 C 2 O 4 tubes when compared with that of plain tubes (11% vs. 4%). No effect of tube type on serum/plasma glucose concentration (p>.05) was noted until two in plain tubes was observed in comparison with plasma measurements after four hour spot collection. Conclusion: Plain tubes are a better option for collection and processing blood in measurement of glucose if the separation is achieved within a reasonable time period. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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