The present study was conducted to evaluate the changes in oxidative stress parameters on experimental infection with in Swiss albino mice. The mice were divided into four groups viz., group I-IV, each group comprising of 15 mice. Group I mice served as healthy control. In Group II mice, oocysts @ 10/os were administered, mice of group III were given dexamethasone @ 30 µg/ml in drinking water whereas group IV mice were given dexamethasone @ 30 µg/ml along with oocysts @ 10/os. Significant changes were seen in oxidative stress parameters which included significant increase in LPO and decrease in levels of SOD, CAT and GSH in liver and intestine in group IV mice at 10th DPI when compared to others indicating an important role played by free radical induced oxidative stress in the development of infection in mice which was clinically characterized by loss of body condition, profuse bloody diarrhoea and peak oocyst shedding intensity occurring at 10th DPI.
The present study was conducted to determine the clinco-haematological effects of a well characterized Cryptosporidium parvum isolate in Swiss albino mice. Sixty female mice were divided into four groups. Group I mice served as healthy control. In group II, C. parvum oocysts were administered orally, mice of group III were given dexamethasone in drinking water whereas group IV mice were given dexamethasone along with C. parvum oocysts. Clinical signs were more severe in immunosuppressed infected mice and observed dullness, depression, inappetance, poor fur condition, progressive weakness, and decrease in body weight. In addition, mice in group IV showed profuse diarrhoea. An overall mortality rate of 7% and 20% was seen in group III and IV animals, respectively. Animals of group IV had significantly lower average body weight as compared to other groups around the time of peak infection with C. parvum which was recorded to be around 10th DPI. Based on severity of clinical disease and oocyst shedding intensity significant leukocytosis along with neutrophilia and lymphocytopenia was observed in group IV mice at 10th DPI as compared to mice in other groups. It was concluded that experimental infection with C. parvum in mice caused a severe clinical disease which peaked around 10th day and was seen to subsequently resolve around 15 DPI.
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