Comparative evaluation of Doolittle, Karl Jenter and Cupkit techniques of Apis mellifera Linnaeus queen bee rearing was done during spring (mid February- mid April 2013) breeding season. The highest acceptance of cell cups (66.00 %), queen cells raising (64.00 %), their sealing (60.67 %) and emergence of gynes (54.67 %) was recorded in Cupkit apparatus. Maximum weight of newly emerged gyne was recorded in Doolittle method in plastic cell cups (212.36 mg), while the mean weight was 184.96 mg in case of Cupkit apparatus. Overall, Cupkit proved to be the best option for queen bee rearing because of its better performance in terms of acceptance of larvae (66.00 %) and the number of successfully produced gynes i.e. 16 queens/colony/cycle of 12 days.
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