The relationship of chronotype differences with dietary habits and health-related outcomes among elderly people is not fully understood, although sex and generation differences are observed in human chronotype. Accordingly, we analysed the association of chronotype (as assessed by the midpoint of sleep) with dietary intake and health-related quality of life (HRQoL) in elderly Japanese women. The subjects in this cross-sectional study were 1618 women aged 65 years and older who were grandmothers or acquaintances of dietetics students. The subjects were classified into quintiles with respect to the midpoint of sleep, from the earliest to the latest quintile. HRQoL was assessed by the Japanese version of the short-form 36-item health survey score. Mental health was assessed by the Center for Epidemiologic Studies Depression (CES-D) Scale. Dietary intake was assessed by a brief-type self-administered diet history questionnaire. A later midpoint of sleep was associated with a lower intake of vitamin D and a higher intake of bread and caffeinated drinks. No correlations were observed between chronotype and other nutrient and food intake. The subjects with a late midpoint of sleep (eveningness) showed poor general health perception (GH) and high CES-D scores. Other HRQoL scores did not differ among groups with different midpoints of sleep. In conclusion, chronotype as assessed by the midpoint of sleep is associated with poor GH and depressive tendencies in elderly Japanese women. Additionally, a few associations were found between dietary intake and chronotype in elderly Japanese women.