Summary
Rhizosheaths function in plant−soil interactions, and are proposed to form due to a mix of soil particle entanglement in root hairs and the action of adhesive root exudates. The soil‐binding factors released into rhizospheres to form rhizosheaths have not been characterised. Analysis of the high‐molecular‐weight (HMW) root exudates of both wheat and maize plants indicate the presence of complex, highly branched polysaccharide components with a wide range of galactosyl, glucosyl and mannosyl linkages that do not directly reflect cereal root cell wall polysaccharide structures. Periodate oxidation indicates that it is the carbohydrate components of the HMW exudates that have soil‐binding properties. The root exudates contain xyloglucan (LM25), heteroxylan (LM11/LM27) and arabinogalactan‐protein (LM2) epitopes, and sandwich‐ELISA evidence indicates that, in wheat particularly, these can be interlinked in multi‐polysaccharide complexes. Using wheat as a model, exudate‐binding monoclonal antibodies have enabled the tracking of polysaccharide release along root axes of young seedlings, and their presence at root hair surfaces and in rhizosheaths. The observations indicate that specific root exudate polysaccharides, distinct from cell wall polysaccharides, are adhesive factors secreted by root axes, and that they contribute to the formation and stabilisation of cereal rhizosheaths.
Due to the significant losses contributed by the secondary flow features, an active flow control system was implemented in a low-pressure turbine linear cascade which consisted of localized endwall jets with small mass ratios to perturb the dominant passage vortex. Benefits included significant area-averaged total pressure loss reduction and improved exit angle deviations which help to open the design envelope to application of high-lift front-loaded blades. This report looks to reveal the impact of steady and pulsed endwall blowing on the secondary flow dynamics. High-speed stereoscopic particle image velocimetry for an in-passage measurement plane was utilized to investigate the time-dependent behavior of key flow features such as the passage vortex. At baseline conditions, the passage vortex is characterized by time-varying oscillatory motion in the pitchwise direction, streamwise undulation, bursting, and fluctuating strength. Upon actuation of endwall jets, some of these defining dynamics of key flow features were greatly affected. A complementary investigation of the endwall jets mounted outside of a turbine environment in order to study the emitted structures at varying conditions was used to explain the observations found in the turbine passage. Insights into the secondary flow responsiveness demonstrated that loss reduction was realized by inducing reduced coherence of the passage vortex. Despite pulsed blowing at discrete frequencies associated with the passage vortex, there was no indication that instability excitation was exploited. Rather, the endwall jets acted as a periodic shape-change to the endwall which weakened the passage vortex and forced it closer to the suction-surface.
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