Endophytic actinomycetes encompass bacterial groups that are well known for the
production of a diverse range of secondary metabolites. Vochysia
divergens is a medicinal plant, common in the “Pantanal” region
(Brazil) and was focus of many investigations, but never regarding its community of
endophytic symbionts. During a screening program, an endophytic strain isolated from the
V. divergens, was investigated for its potential to show biological
activity. The strain was characterized as Microbispora sp. LGMB259 by
spore morphology and molecular analyze using nucleotide sequence of the 16S rRNA gene.
Strain LGMB259 was cultivated in R5A medium producing metabolites with significant
antibacterial activity. The strain produced 4 chemically related
β-carbolines, and 3 Indoles. Compound
1-Vinyl-β-carboline-3-carboxylic acid displayed potent activity
against the Gram-positive bacterial strains Micrococcus luteus NRRL
B-2618 and Kocuria rosea B-1106, and was highly active against two human
cancer cell lines, namely the prostate cancer cell line PC3 and the non-small-cell lung
carcinoma cell line A549, with IC50 values of 9.45 and 24.67 µM,
respectively. 1-Vinyl-β-carboline-3-carboxylic acid also showed
moderate activity against the yeast Saccharomyces cerevisiae ATCC204508,
as well as the phytopathogenic fungiPhyllosticta citricarpa LGMB06 and
Colletotrichum gloeosporioides FDC83.
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