Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2.5% NaOCl for 5 days or kept in PBS. Tooth slice conditioned media were prepared and used for TGF-b1 ELISA and migration assays. Culture medium with different concentrations of recombinant human TGF-b1 (0.5, 1.0, 5.0 or 10.0 ng/mL) was also tested by migration assay. The data were evaluated by ANOVA and Tukey's test. Optical density values corresponding to media conditioned by tooth slices either containing or not containing the predentin layer and treated with 10% EDTA were statistically greater than the other groups and close to 1 ng/mL. Increased rates of migration toward media conditioned by tooth slices containing the predentin layer and treated with PBS, 10% EDTA or 2.5% NaOCl were observed. Recombinant human TGF-b1 also stimulated migration of SHED, irrespective of the concentration used. EDTA may be considered an effective extractant of TGF-b1 from the dentin matrix. However, it does not impact SHED migration, suggesting that other components may account for the cell migration.

Pasture and crop lands restrict seed dispersal near remnant forest fragments, especially by restricting the movements of dispersal agents and limiting propagule dispersal. Some factors can improve seed dispersal in open areas, such as the presence of high numbers of isolated trees in close proximity to forest fragments. We sought to determine if: (i) the structural characteristics and (ii) densities of isolated trees in pasture lands, and (iii) their distances from the forest fragments, infl uence seed dispersal. We installed 18 seed traps in each of six pastures (total=108 traps) bordering forest fragments distributed over 6 distance classes from the forest edges (0, 5, 10, 20, 40 and 80 m). We determined the characteristics of the plants surrounding the traps. GLM and GLMer analyses were performed and the best model was selected by AIC. We collected 8162 seeds (4722 anemochorous, 3304 epizoochorous, 72 autochorous, and 64 endozoochorous) belonging to 26 species. Our results showed that plants with high crown coverage close to forest fragments and at high densities in the pastures increased seed dispersal. Th ese results may aid future restoration of pasture lands by improving seed dispersal in this harsh habitat and promoting better connectivity between forest fragments.

Purpose To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). Methodology Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). Results The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. Conclusion The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA.

Objective: to evaluate the survival, in five years, of women with breast cancer. Method: quantitative, descriptive, exploratory, documental study, consisting of 62 charts of women attended in an Extension Project. Data were analyzed by Fisher's Exact Test, Kaplan Meier Curve and software R. Results: 61.29% (n = 38) had breast cancer aged 50-69 years; 35.49% (n = 22) were metastasized; 37.1% (n = 23) of them died, and the greatest cause was 87% breast cancer (n = 54). The survival rate was 80%, with an average time of 11.27 years. Women who had metastases had 3.67 more chances of dying (p = 0.00658), therefore, they had a shorter survival (p = 00171). Conclusion: the incidence of breast cancer was higher in patients aged 50-69 years, which is in agreement with the coverage of screening programs recommended by the Ministry of Health. Descriptors: Breast neoplasms; Survival analysis; Oncology Nursing. RESUMOObjetivo: avaliar a sobrevida, em cinco anos, de mulheres com câncer de mama. Método: estudo quantitativo, descritivo, exploratório, documental, constituído de 62 prontuários de mulheres atendidas em um Projeto de Extensão. Os dados foram analisados pelo Teste Exato de Fisher, Curva de Kaplan Meier e software R. Resultados: 61,29% (n=38) tiveram câncer de mama com idade entre 50-69 anos; 35,49% (n=22) foram acometidas por metástase; 37,1% (n=23) delas morreram, sendo que a maior causa foi o câncer de mama 87% (n=54). A sobrevida foi de 80%, com tempo médio de 11,27 anos. As mulheres que tiveram metástase possuíam 3,67 mais chances de morrer (p=0,00658), por isso, elas tiveram uma sobrevida menor (p= 00171). Conclusão: a incidência do câncer de mama foi maior em pacientes com faixa etária de 50-69 anos, o que está em acordo com a cobertura de programas de rastreamento preconizados pelo Ministério da Saúde. Descritores: Neoplasias da Mama; Análise de Sobrevida; Enfermagem Oncológica. RESUMENObjetivo: evaluar la supervivencia, en cinco años, de mujeres con cáncer de mama. Método: estudio cuantitativo, descriptivo, exploratorio, documental, constituido de 62 prontuarios de mujeres atendidas en un Proyecto de Extensión. Los datos fueron analizados por la Prueba Exacta de Fisher, Curva de Kaplan Meier y software R. Resultados: 61.29% (n = 38) tuvieron cáncer de mama con edad de 50-69 años; El 35.49% (n = 22) fueron acometidas por metástasis; 37.1% (n = 23) de ellas murieron, siendo que la mayor causa fue el cáncer de mama 87% (n = 54). La sobrevida fue del 80%, con tiempo promedio de 11.27 años. Las mujeres que tuvieron metástasis tenían 3.67 más veces de morir (p = 0.00658), por lo, que tuvieron una supervivencia menor (p = 00171). Conclusión: la incidencia del cáncer de mama fue mayor en pacientes con edades de 50-69 años, lo que está en acuerdo con la cobertura de programas de rastreo preconizados por el Ministerio de Salud. Descriptores:

Introdução: O correto manejo de um dente avulsionado é crucial para o sucesso do tratamento. Objetivo: Avaliar qual melhor estratégia de ensino (palestra, folder e banner) para pais/responsáveis sobre avulsão dentária e conduta de urgência. Metodologia: A população deste estudo foi de pais ou responsáveis de crianças com 6 a 9 anos de três escolas privadas. Primeiramente foi aplicado um questionário para verificar os conhecimentos dos participantes do estudo sobre avulsão dentária. Subsequente, em cada escola, uma estratégia de ensino foi realizada. Após 30 dias aplicou-se novamente o mesmo questionário. Para a análise estatística foi realizada a comparação das proporções de X e de Y (p≤0,05). Resultados: A palestra se mostrou inviável devido à ausência dos pais ou responsáveis, no evento previamente agendado. Porém isso não comprova que ela seja ineficaz. Verificou-se um conhecimento satisfatório após a leitura do banner e do folheto, porém não houve significância estatística.  Em relação ao melhor meio de armazenamento do dente avulsionado o banner apresentou-se estatisticamente mais eficaz que o folder (p < 0,05). Conclusão: O banner e o folder são capazes de transmitir informações, aos pais/responsáveis, de como agir diante uma avulsão dentária. Assim, indivíduos leigos podem tornar mais aptos quando tiverem noções, mesmo básicas, sobre os cuidados após o trauma e com dente avulsionado.