The goal of this study was to show that nonviral gene transfection technology can be used to genetically modify neuroblastoma cells with immune stimulatory molecules, and that the modified cells can generate an antitumor immune response. The authors found that an electroporation-based gene transfection method, nucleofection, could be used to modify mouse AGN2a (an aggressive variant of Neuro-2a) neuroblastoma cells to simultaneously express as many as four different immune stimulatory molecules encoded by separate plasmid vectors. Within 18 hours after nucleofection, greater than 60% of the cells typically expressed the transfected gene products, and the percentages of cells expressing the products often exceeded 96%. High levels of plasmid in cell nuclei immediately after nucleofection documented instantaneous availability of gene vectors to the transcriptional machinery. AGN2a cells nucleofected to express the co-stimulatory molecules CD80 and CD86 expressed higher levels of these molecules than cells that had been permanently transfected with these same plasmid vectors, and the nucleofected cells were as effective as the permanently transfected cells at inducing an antitumor response in vivo in a tumor prevention model. AGN2a cells nucleofected with four separate plasmid vectors encoding CD54, CD80, CD86, and CD137L induced a T-cell immune response in vitro and served as a potent tumor vaccine in the tumor prevention model. These data show that transient transfection using a nonviral based method, nucleofection, can be used to rapidly generate novel cell-based tumor vaccines.

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<p>Servant leader leading by not pursuing personal interest but striving to do the best for the organization and the people they lead. Servant leader can improve job satisfaction and trust of the people they lead. A good organizational climate also can triggers job satisfaction and organizational trust of the member towards the organization. This research was conducted with the aim to know the positive effect of servant leadership, organizational climate, and job satisfaction on organizational trust of the teacher in Sekolah Lentera Harapan Curug. This study uses quantitative approach with the subject of research amounted to 50 respondents. Path analysis and descriptive statistic were used to analyze the data collected. Result shows that (1) servant leadership has positive effect on job satisfaction; (2) organizational climate has positive effect on job satisfaction; (3) servant leadership has positive effect on organizational trust; (4) organizational climate has positive effect on organizational trust; and (5) job satisfaction has positive effect on organizational trust.</p><p><strong>BAHASA INDONESIA ABSTRACT: </strong>Seorang pemimpin pelayan menjalankan kepemimpinan dengan tidak mengejar kepentingan pribadi tetapi berusaha untuk mengupayakan yang terbaik bagi kemajuan organisasi dan orang-orang yang dipimpinnya. Kepemimpinan pelayan dapat mendorong kepuasan dan kepercayaan orang yang dipimpinnya. Selain itu, iklim organisasi yang baik juga turut memicu kepuasan dan kepercayaan anggota terhadap organisasi. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh <em>servant leadership, </em>iklim organisasi, dan kepuasan kerja terhadap kepercayaan organisasi guru di Sekolah Lentera Harapan Curug. Penelitian ini menggunakan pendekatan kuantitatif dengan subjek penelitian sejumlah 50 responden. Metode pengolahan data menggunakan analisis jalur (<em>path analysis</em>) dan metode statistik deskriptif. Dari hasil penelitian diperoleh kesimpulan bahwa (1) <em>servant leadership </em>berpengaruh positif terhadap kepuasan kerja; (2) iklim organisasi berpengaruh positif terhadap kepuasan kerja; (3) <em>servant leadership </em>berpengaruh positif terhadap kepercayaan organisasi; (4) iklim organisasi berpengaruh positif terhadap kepercayaan organisasi; dan (5) kepuasan kerja berpengaruh positif terhadap kepercayaan organisasi.</p>