Creamy friable calli were induced from meristems (scalps) of proliferating shoots of plantain (Musa sp.) cv. Spambia (genome AAB) incubated on a semi-solid modified Murashige and Skoog (MS) medium supplemented with 4.5 lM 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 lM zeatin. About 25% of shoot-tip explants formed scalps, and about 98% of scalps developed embryogenic calli. Small dense aggregates of cells, were obtained when these calli were transferred to liquid MS medium supplemented with 4.5 lM 2,4-D and 1.0 lM zeatin. Upon transfer to semi-solid MS medium of the same composition as described above, aggregates of cells formed somatic embryos. In the presence of 2.5 lM abscisic acid (ABA), maturation of somatic embryos was 2.6-fold higher than that of control (lacking ABA), and regardless of the type of cytokinin used in the medium. Upon transfer to MS medium supplemented with 1.25 lM 6-benzyladenine (BA), 80% of germinated embryos developed into plantlets.
Viruses cause great losses in agriculture. One of the major viruses affecting strawberry is Strawberry latent ringspot virus (SLRSV) and Strawberry mild yellow edge potexvirus (SMYEPV). Propagation via tissue culture is always accompanied with genetic instability. To produce a virus-free and genetically stable plant of commercial strawberry cultivar Hadas, a regeneration protocol was established using repeated shoot meristem from in vitro grown plant. Enzyme-linked immunosrbent assay (ELIZA) was used for virus's detection and Random amplified polymorphic DNA (RAPD) analysis was used to check for genetic stability of propagated plants. Results indicated that MS medium supplemented with BAP (2mg/ l) and GA3 (1 mg/l) gave the maximum number of shoots (5 shoots/explant) with maximum average shoot length of 4.3cm. For rooting, MS medium supplemented with IBA at concentration of 1 mg/ l gave the best results (7 roots /explant) and with average root length of 4.5cm. ELIZA results showed that repeated
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