Traditional medicine has a great impact on aboriginal heal thin Algerian Sahara and particularly in Hoggar. It is based on the use of local plants.This study is based on phytochemical screening. Antioxidant activity evaluation by two methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH): bioautography and spectrophotometric assay, and microbiological activity of twelve plants species from nine families sampled in Hoggar.The antioxidant activity revealed by the bioautography (DPPH) showed a positive activity on aqueous extracts of ten species among the twelve assayed. Both species Lavandula pubescens subesp. antinea and Mentha longifolia (Lamiaceae) have reported a significant activity compared to the others.The comparison of antiradical activity bybioautography and phytochemical study by TLC showed the presence of cardiotonic glycosides, saponins and flavonoids which could be responsible of antioxidant activity or some species.The antimicrobial activity assay showed that plant aqueous extracts were inefficient on the majority of tested strains except for Balanites aegyptica extract on Bacillus subtilis ATCC6633. Moreover, remarkable antifungal activity of Aerva javanica aqueous extracts on four fungal strains such as Candida albicans C200, Aspergillus flavus AF1, Aspergillus carbonarius M333, Fusarium culmorum FC1was observed.
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