Background:
The vessel restenosis is related to the inflammatory events in subendothelial space.
It is proposed that the inflammatory agents affect the prostaglandin synthesis pathway. In this
study, we investigated the COX-1, COX-2, PTGDS and miRNA-520a-5p expression levels and
the serum 15-Deoxy-∆
12,14
–PGJ2 metabolite values in patients with the stenosed and re-stenosed
vessels. Furthermore, the associations between genes and miR-520 were evaluated in the monocyte
transfection studies.
Methods:
The subjects (n=60) were included three groups; healthy subjects (control (stenosis <
5%), stent no restenosis (SNR, restenosis < 5%) and in-stent restenosis (ISR, restenosis > 70%)).
The miRNA and gene expression levels were measured by RT-qPCR technique. 15-Deoxy-∆
12,14
–PGJ2 values were measured by the ELISA technique. The miR-520 was transfected into
myocytes using PEI polymer.
Results:
The monocyte COX-1, COX-2 and PTGDS gene expression levels and the serum 15-
Deoxy-∆
12,14
–PGJ2 values increased significantly in the patients. Furthermore, the miR-520
correlated conversely with the COX-1, and PTGDS gene expression levels.
Conclusion:
The results showed that the PGD2 synthesis pathway is active in the patients and,
miR-520 may be involved in the function of this pathway.
Background:The integrin family receptors stimulate the cellular proliferation and migration through
the focal adhesion pathway by the activation of PTK2, VASP and TSP1 proteins. The purpose of this study was
to investigate the integrin-ligated motifs through the activation of focal adhesion pathway.Methods:A chimeric peptide was predicted from the integrin-mediated ligands by bioinformatics tools. The
VSMCs were treated with the chimeric peptide and simvastatin. The PTK2, VASP and TSP1 protein and gene
expression levels were measured by RT-qPCR and Western Blotting techniques, respectively. AutoDock Tools
were used for the docking technique.Results:The PTK2, VASP and TSP1 protein expression levels increased significantly in the VSMCs treated with
chimeric peptide in conversely with the effects of simvastatin. The docking results suggested two motifs in the
chimeric peptide.Conclusion:In conclusion, the chimeric peptide activated the focal adhesion pathway. The motifs 1 and 2 may be
directly involved in the transduction of signal by integrin family receptors.
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