A Peptide Construct Mediates Focal Adhesion Pathway Through the Activation of Integrin Receptor

Khosravi, Mohsen; Kakavandi, Naser; Rezaee, Shima; Shabani, Mohammad; Najafi, Mohammad

doi:10.2174/1381612826666200311125325

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…Platelets could interact with integrin . Except for the interactions mentioned above, integrin activation is motivated by intracellular domain binding proteins, which were partly from focal adhesion. − Focal adhesion can also regulate the connection with the ECM, which is an irreplaceable part of cell adhesion, mobility, migration, angiogenesis, and proliferation . Regarding the GO and KEGG results, we could primarily assume that integrin, platelets, adhesion, and their correlated processes may affect HTV pathogenesis and HAVS progression.…”

Section: Discussionmentioning

confidence: 99%

Vinculin Identified as a Potential Biomarker in Hand-Arm Vibration Syndrome Based on iTRAQ and LC–MS/MS-Based Proteomic Analysis

et al. 2023

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Local vibration can induce vascular injuries, one example is the hand-arm vibration syndrome (HAVS) caused by hand-transmitted vibration (HTV). Little is known about the molecular mechanism of HAVS-induced vascular injuries. Herein, the iTRAQ (isobaric tags for relative and absolute quantitation) followed by liquid chromatography−tandem mass spectrometry (LC−MS/MS) proteomics approach was applied to conduct the quantitative proteomic analysis of plasma from specimens with HTV exposure or HAVS diagnosis. Overall, 726 proteins were identified in iTRAQ. 37 proteins upregulated and 43 downregulated in HAVS. Moreover, 37 upregulated and 40 downregulated when comparing severe HAVS and mild HAVS. Among them, Vinculin (VCL) was found to be downregulated in the whole process of HAVS. The concentration of vinculin was further verified by ELISA, and the results suggested that the proteomics data was reliable. Bioinformative analyses were used, and those proteins mainly engaged in specific biological processes like binding, focal adhesion, and integrins. The potential of vinculin application in HAVS diagnosis was validated by the receiver operating characteristic curve.

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Section: Discussionmentioning

confidence: 99%

Vinculin Identified as a Potential Biomarker in Hand-Arm Vibration Syndrome Based on iTRAQ and LC–MS/MS-Based Proteomic Analysis

et al. 2023

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“…Up-regulation of genes linked to this pathway demonstrated that the barn feeding strategy enhanced the interactions between ECM and membrane receptors and stimulated the activation of intracellular signaling pathway. The focal adhesion pathway is closely associated with cellular proliferation due to the activation of focal adhesion kinase 1 ( PTK2 ) which is central to the focal adhesion signaling pathway (Khosravi et al, 2020 ). As the primary structural protein in the extracellular space, collagen is the most abundant protein in mammals and is encoded by the collagen gene family.…”

Section: Discussionmentioning

confidence: 99%

Different feeding strategies can affect growth performance and rumen functions in Gangba sheep as revealed by integrated transcriptome and microbiome analyses

et al. 2022

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Due to the harsh environment in the Tibetan Plateau, traditional grazing greatly limits the growth potential of local animals and causes severe ecosystem degradation. This is an urgent issue to be solved, which requires alternative strategies for grazing animals in the Tibetan alpine pastoral livestock systems. This study aimed to investigate the effects of different feeding strategies on growth performance and ruminal microbiota-host interactions in the local breed of sheep (Gangba sheep). Thirty 9-month old Gangba sheep (n = 10 per group) were assigned to natural grazing (G), semi-grazing with supplementation (T), and barn feeding (F) groups (supplementation of concentrate and oat hay) based on body weight. At the end of the experiment (75 d), all sheep were weighed, rumen fluid was obtained from six sheep per group, and ruminal epithelium was obtained from 3 sheep per group. The results showed that: (1) Compared with the G and T groups, the F group significantly increased dry matter intake, average daily gain, and feed conversion ratio of animals. Additionally, Gangba sheep in the F group had higher concentrations of ruminal short-chain volatile fatty acids (VFAs), especially propionate and butyrate (P <0.05) than sheep in the G and T groups. (2) The principal coordinates analysis indicated a significant difference in bacterial composition among different feed strategies. More specifically, the relative abundance of propionate (unidentified F082 and Succiniclasticum) and butyrate-producing (Eubacterium_coprostanoligenes_group) genera were also observed to be increased in the F group, in which unidentified F082 was identified as a differential biomarker among the three groups according to linear discriminant analysis effect size analysis. (3) The dynamics of the rumen epithelial transcriptome revealed that ECM-receptor interactions, focal adhesion, and PI3K-Akt signaling pathways, which are critical in mediating many aspects of cellular functions such as cell proliferation and motility, were upregulated in the F group. In conclusion, under harsh conditions in the Tibetan alpine meadow, barn feeding increased ruminal VFAs concentrations (especially propionate and butyrate), which stimulated gene expression related to cell proliferation in rumen epithelium, appearing to be superior to natural grazing and semi-grazing in gaining body weight of the local Gangba sheep.

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A Peptide Construct Mediates Focal Adhesion Pathway Through the Activation of Integrin Receptor

Cited by 2 publications

References 24 publications

Vinculin Identified as a Potential Biomarker in Hand-Arm Vibration Syndrome Based on iTRAQ and LC–MS/MS-Based Proteomic Analysis

Vinculin Identified as a Potential Biomarker in Hand-Arm Vibration Syndrome Based on iTRAQ and LC–MS/MS-Based Proteomic Analysis

Different feeding strategies can affect growth performance and rumen functions in Gangba sheep as revealed by integrated transcriptome and microbiome analyses

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