Mobile learning game has been widely used for encouraging students whenever and wherever. However, several studies have indicated that without properly incorporating students' personalised characteristic and learning assistance mechanism into the digital game-based learning environment might fail to motivate students learning and to help them acquire knowledge. To address this issue, this study proposes an integrated learning diagnosis and mastery learning mechanism to generate appropriate gaming stage according to the conceptual levels of an individual student in a pre-test and monitor learning during the gaming process. A mobile game-based learning system was developed on the general science course in four Thai elementary schools with 282 students to evaluate its effectiveness. The experimental results showed that the students achieved the concepts of force and motion through the proposed approach. The findings also highlight relationships between the proposed game and the conventional game in terms of students' achievement clusters, performance, and perceptions.
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