<p>The aim of the present study was to investigate the anticancer activity of <em>Drimia nagarjunae</em> (Liliaceae) extracts against Colo205 human colon cancer cell lines by SRB assay for the first time. The bulbs and leaves of the plant were sequentially extracted using solvents with increasing polarities (hexane> chloroform>ethyl acetate>methanol>water). Ethyl acetate and chloroform bulb extracts showed potent anticancer activity compared to standard, adriamycin. Both the extracts exhibited total growth inhibition of cell at 20.1 µg/mL and 32.1 µg/mL whereas adriamycin shown 33.1 µg/mL and 50% lethal concentration was found to be 61.5 µg/mL and >80 µg/mL respectively. The active extracts were subjected to GC-MS analysis for identification of phytocompounds and it showed seven and ten major compounds respectively. Therefore, the present study demonstrated that <em>D. nagarjunae</em> can be a promising candidate as an anticancer agent.</p><p> </p>
Syzygium aromaticum, a popular ethanomedicinal herb, possesses quorum quenching and inhibition of virulence factors of Pseudomonas aeruginosa. The objective of the present study was to partially purify and study quorum sensing inhibition of S.aromaticum methanolic extract using in vitro and in silico docking studies. S. aromaticum was extracted with methanol using Soxhlet apparatus and was subjected to TLC-bioautography using Chromobacterium violaceum as reporter strain. The TLC purified fraction was subjected to biofilm inhibition on P. aeruginosa and GC-MS analysis. The identified compounds from GC-MS were docked with LasI and EsaI proteins. TLC-bioautography analysis showed clear zone of inhibition at the bioactive fraction (R f =0.78) and also exhibited dose dependent (50-200 µg/mL) biofilm inhibition on P. aeruginosa. GC-MS analysis of active fraction revealed six major compounds viz. 4-hydroxy-3-methoxy-benzaldehyde, 1-phenoxy ethanol, eugenol, chavicol, p-hydroxyacetophenone and 4-azido-phenol that may account for the antibiofilm activity. These compounds were studied for their in silico binding patterns with quorum sensing regulated proteins, LasI and EsaI. Among all, chavicol formed three hydrogen bonds with LasI (-6.20 kcal/mol) and three hydrogen bonds with EsaI (-4.36 kcal/mol), indicating best interaction with targeted protein binding sites. The current study demonstrated that the
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