This experiment was conducted to investigate the efficacy of different levels of biotin on broilers ' performances, protein and fat apparent digestibility and carcass characteristics. The study was conducted at University of Duhok, College of agriculture, Animal House Complex. A total of 160-day-old Ross 308 randomly assigned to 4 treatments with four replicate pens per treatment and 10 birds each pen. Treatments were control diet without additive and three levels of biotins which are biotin 1, biotin 2 and biotin 3 at concentrations 1000, 1500 and 2000 mg/10L of drinking water respectively during the whole rising period. The results showed that different levels of biotin increased body weight gain (WG) and biotin2 and biotin 3 improved Feed conversion ratio (FCR) compared to control group at day 35. Adding different levels of biotin in drinking water had significantly higher protein digestibility over non-treated group. In conclusion, adding biotin to broiler water had positive effect on WG, FCR and protein digestibility.
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