The tomato is an excellent model for studies of plants bearing berry-type fruits and for experimental studies of the Solanaceae family of plants due to its conserved genetic organization. In this study, a comprehensive mutant tomato population was generated in the background of Micro-Tom, a dwarf, rapid-growth variety. In this and previous studies, a family including 8,598 and 6,422 M2 mutagenized lines was produced by ethylmethane sulfonate (EMS) mutagenesis and γ-ray irradiation, and this study developed and investigated these M2 plants for alteration of visible phenotypes. A total of 9,183 independent M2 families comprising 91,830 M2 plants were inspected for phenotypic alteration, and 1,048 individual mutants were isolated. Subsequently, the observed mutant phenotypes were classified into 15 major categories and 48 subcategories. Overall, 1,819 phenotypic categories were found in 1,048 mutants. Of these mutants, 549 were pleiotropic, whereas 499 were non-pleiotropic. Multiple different mutant alleles per locus were found in the mutant libraries, suggesting that the mutagenized populations were nearly saturated. Additionally, genetic analysis of backcrosses indicated the successful inheritance of the mutations in BC1F2 populations, confirming the reproducibility in the morphological phenotyping of the M2 plants. To integrate and manage the visible phenotypes of mutants and other associated data, we developed the in silico database TOMATOMA, a relational system interfacing modules between mutant line names and phenotypic categories. TOMATOMA is a freely accessible database, and these mutant recourses are available through the TOMATOMA (http://tomatoma.nbrp.jp/index.jsp).
Salinity stress enhances sugar accumulation in tomato (Solanum lycopersicum) fruits. To elucidate the mechanisms underlying this phenomenon, the transport of carbohydrates into tomato fruits and the regulation of starch synthesis during fruit development in tomato plants cv. ‘Micro-Tom’ exposed to high levels of salinity stress were examined. Growth with 160 mM NaCl doubled starch accumulation in tomato fruits compared to control plants during the early stages of development, and soluble sugars increased as the fruit matured. Tracer analysis with 13C confirmed that elevated carbohydrate accumulation in fruits exposed to salinity stress was confined to the early development stages and did not occur after ripening. Salinity stress also up-regulated sucrose transporter expression in source leaves and increased activity of ADP-glucose pyrophosphorylase (AGPase) in fruits during the early development stages. The results indicate that salinity stress enhanced carbohydrate accumulation as starch during the early development stages and it is responsible for the increase in soluble sugars in ripe fruit. Quantitative RT-PCR analyses of salinity-stressed plants showed that the AGPase-encoding genes, AgpL1 and AgpS1 were up-regulated in developing fruits, and AgpL1 was obviously up-regulated by sugar at the transcriptional level but not by abscisic acid and osmotic stress. These results indicate AgpL1 and AgpS1 are involved in the promotion of starch biosynthesis under the salinity stress in ABA- and osmotic stress-independent manners. These two genes are differentially regulated at the transcriptional level, and AgpL1 is suggested to play a regulatory role in this event.
Steep gradients of temperature and density, called cold fronts, are observed by Chandra in a leading edge of subclusters moving through the intracluster medium (ICM). The presence of cold fronts indicates that thermal conduction across the front is suppressed by magnetic fields. We carried out three-dimensional magnetohydrodynamic (MHD) simulations including anisotropic thermal conduction of a subcluster moving through a magnetically turbulent ICM. We found that turbulent magnetic fields are stretched and amplified by shear flows along the interface between the subcluster and the ambient ICM. Since magnetic fields reduce the efficiency of thermal conduction across the front, the cold front survives for at least 1 Gyr. We also found that a moving subcluster works as an amplifier of magnetic fields. Numerical results indicate that stretched turbulent magnetic fields accumulate behind the subcluster and are further amplified by vortex motions. The moving subcluster creates a long tail of ordered magnetic fields, in which the magnetic field strength attains a value of ¼ P gas /P mag P 10.
We have carried out near-IR/optical observations to examine star formation toward a bright-rimmed cometary globule (BRC37) facing the exciting star(s) of an H ii region (IC1396) containing an IRAS source, which is considered to be an intermediate-mass protostar. With slitless spectroscopy we detected ten Hα emission stars around the globule, six of which are near the tip of the globule and are aligned along the direction to the exciting stars. There is evidence that this alignment was originally toward an O9.5 star, but has evolved to align toward a younger O6 star when that formed. Near-IR and optical photometry suggests that four of these six stars are low-mass young stellar objects (YSOs) with masses of ∼0.4 M . Their estimated ages of ∼1 Myr indicate that they were formed at the tip in advance of the formation of the IRAS source. Therefore, it is likely that sequential star formation has been taking place along the direction from the exciting stars toward the IRAS source, due to the UV impact of the exciting star(s). Interestingly, one faint, Hα emission star, which is the closest to the exciting star(s), seems to be a young brown dwarf that was formed by the UV impact in advance of the formation of other YSOs at the tip.
We show three-dimensional numerical simulations in which stars form sequentially in a Ðlamentary molecular cloud. The star formation is triggered by expansion of an H II region. The H II region is distant from the Ðlamentary cloud at the initial stage. As it expands, it interacts with the Ðlamentary cloud. The cloud is pinched and separated into two parts. Subsequently, the gravitational instability is induced to form two cores of the Ðrst generation along the Ðlament axis in a typical model. The separation of the two cores is several times larger than the Ðlament diameter. It is comparable to the wavelength of the fastest growing fragmentation mode. The Ðrst-generation cores become isolated, and Ðlamentary clouds shorten to widen the separation. New cores of second generation form at the edges of the shortened Ðlamentary clouds. This core formation is recursive, and our model shows sequential star formation triggered by an expanding H II region. The age di †erence is several times that of the dynamical timescale between the Ðrst-and second-generation cores. This sequential star formation is similar to that observed in the Ðlamentary cloud associated with the H II region NGC 2024. Our Ðrst-generation cores correspond to FIR 4 and FIR 5, while the second-generation cores correspond to FIR 3 and FIR 6.
Recent Chandra observations of clusters of galaxies revealed the existence of a sharp ridge in the X-ray surface brightness where the temperature drops across the front. This front is called the cold front. We present the results of twodimensional magnetohydrodynamic simulations of the time evolution of a dense subcluster plasma moving in a cluster of galaxies. Anisotropic heat conduction along the magnetic field lines is included. In the models without magnetic fields, the numerical results indicate that the heat conduction from the hot ambient plasma heats the cold dense plasma of the subcluster and diffuses out the cold front. When magnetic fields exist in a cluster of galaxies, however, cold fronts can be maintained because the heat conduction across the magnetic field lines is suppressed. We found that, even when the magnetic fields in a cluster of galaxies are disordered, heat conduction across the front is restricted because the magnetic field lines are stretched along the front. Numerical results reproduced the X-ray intensity distribution observed in the A3667 cluster of galaxies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.