Facultatively chemolithotrophic thiobacilli, Thiobacillus perometabolis THI 022, Thiobacillus sp. A2, Thiobacillus novellus, Thiobacillus organoparus and Thiobacillus acidophilus, had ubiquinones with 10 isoprene units (Q-10) (group I). These species also had [octadecenoic acid (18 : 1) + cyclopropane acid of CI9 (19, , , )] as major non-hydroxylated fatty acids. The organisms, except T. novellus, contained 3-hydroxydecanoic acid (3-OH 10 : 0) or 3-hydroxytetradecanoic acid (3-OH 14 : 0) as hydroxy fatty acids. DNA base compositions of these species were 63 to 68 mol% G + C. Other facultatively chemolithotrophic thiobacilli, including T. perometabolis THI 023, Thiobacillus delicatus and Thiobacillus intermedius with or without [ 18 : 1 + 19, , , ] as major non-hydroxylated fatty acids. The species of group I11 contained 3-OH 12:O and some had 3-OH 10:0 when grown at neutral pH and 3-OH 14 :O in acidophilic conditions. DNA base compositions of these organisms ranged from 5 1 to 64 mol% G + C. Chemotaxonomic characteristics would seem to be useful for the identification and the classification of thiobacilli.

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The produaion und svxction of plrrminogcn in cuhurcd rat brdin microalia was investigated. Ur&inaredcpcndcnr carinolytic activity was dctcctcd by nymwraphy in mirroylhl conditioned malium with tt molecular weight of about 90 kDa. The 90&l& protein WBT also dctcstcd by Western blotting with anti-rat plrrrminovn antiserum in the non.rcduciny condifion. Immunaprccipitauion with plusminogcn antirrum following ("S]mcthioninc lirbclling rcvcalcd that the plasminogcn dcectrd in mkroglirl condirioncd medium is synthbcd in micro@. Ths amount of plasminogen in ~lre condhloncd medium WYI increased by stimulation with lipopolywccharidc. Thcsc rcsultr show that cultured misroar preducc plasminoycn and secrctc it into the culture medium.

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