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We describel iquid-liquide xtraction of short and single-stranded DNA using reversem icelles (water-in-oilm icroemulsions)w ith aD NA-surfactant. AD NA-surfactant facilitated extraction of single-stranded target DNA, transporting at arget DNA from an aqueous phase to an organic phase via DNA hybridization.R eversem icelles acted as nanoscale containers that can encapsulate the DNA-surfactant/target DNA complex in the organic phase. Various parameters (such as co-surfactant concentration, molecular design of the DNA-surfactant, and length of the target DNA)d uring extraction were investigated to optimize the conditions. Finally,athrombin-binding DNA aptamerw as extracted into an organic phase using the reverse micellar system described and then successfully back extracted into an aqueous phase. At hrombin-binding assay demonstrated that the back-extracted DNA aptamer retained its intrinsic binding activity.

We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.

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