Salterns are hypersaline environments that are inhabited by diverse halophilic microorganisms, including fungi. In this study, we isolated a fungal strain SK1-1 from a saltern in the Republic of Korea, which was identified as Asperillus reticulatus. This is the first reported saline-environment-derived A. reticulatus that belongs to the Aspergillus penicillioides clade and encompasses xerophilic fungi. SK1-1 was halophilic, obligately requiring NaCl for growth, with a maximum radial growth of 6%–9% (w/v) NaCl. To facilitate the biotechnological application of halophilic fungi, we screened the SK1-1 strain for proteolytic activity. Proteases have widespread applications in food processing, detergents, textiles, and waste treatment, and halophilic proteases can enable protein degradation in high salt environments. We assessed the proteolytic activity of the extracellular crude enzyme of SK1-1 using azocasein as a substrate. The crude protease exhibited maximum activity at 40–50 °C, pH 9.5–10.5, and in the absence of NaCl. It was also able to retain up to 69% of its maximum activity until 7% NaCl. Protease inhibitor assays showed complete inhibition of the proteolytic activity of crude enzymes by Pefabloc® SC. Our data suggest that the halophilic A. reticulatus strain SK1-1 produces an extracellular alkaline serine protease.
In this study, for integrated management and inter-exchange of operational data generated by ships and land-side information on safe and business, a vessel communication system with modular functions was designed that applied high efficiency compression, least-cost algorithms and Inmarsat FBB connection automation system. Performance test at the KTsat Kumsan satellite earth station; system was found to delivered an average transfer speed of 7 kB/S, which was significant improvement from the existing commercial product's average speed of 5 kB/S. It also delivered twice the efficiency of the existing product in terms of compression rate and transfer of the most widely used office files in maritime businesses.
In this study, has designed the ship application system for efficient data integration management of onboard ship and shore application/service utilizing data collected onboard ship, and has implemented the module. In order to supports onboard ship and shore service utilizing onboard ship collected data and provide a easy to access among individual devices, the ship application system applied the XML structure of ISO 16425 and the data sharing system model discussed in IALA, and the common module for system operation, a windows service for data collection/integral management, and web service module for management has been implemented.
: The purpose of hull stress monitoring system(HSMS) is to lead hull structure to be safer at lower cost. We proposed a hull stress monitoring system for leisure boats using fiber-optic bragg grating(FBG) strain sensor. Fiber optic sensors are well suited for structure monitoring system, due to their ability to withstand harsh environments, immunity to electromagnetic interference, and reduce cabling installation cost when employing wavelength multiplexing. This paper presents an overview of current research and design of hull stress monitoring system for leisure boats.
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