Intestinimonas butyriciproducens gen. nov., sp. nov., a butyrate-producing bacterium from the mouse intestine

In.tes.ti.ni.mo'nas. L. neut. n. intestinum , gut, intestine; L. fem. n. monas , a monad, unit; N.L. fem. n. Intestinimonas , a unit (bacterium) isolated from the intestine. Firmicutes / Clostridia / Clostridiales / Ruminococcaceae / Intestinimonas Gram‐positive, strictly anaerobic bacteria, chemoorganotrophic. Grow in a temperature range of 25–45°C, are straight fusiform rods. I. butyriciproducens forms terminal spores. Motility has not been observed. The major fermentation products in Wilkins–Chalgren anaerobe (WCA) broth are butyrate and acetate. Growth on hexose sugars is enhanced by addition of acetate. The cellular fatty acids are mostly saturated (91.9%), including C 14 :0 methyl ester (33.1%) and C 18 :0 dimethyl acetal (23.0%) as the two major fatty acids. Respiratory quinones have not been detected. Comparative analysis of the 16S rRNA gene sequences revealed that members of the genus Intestinimonas share less than 95% similarity with their closest cultured relatives Flavonifractor plautii and Pseudoflavonifractor capillosus and fall within Clostridium Cluster IV. The type strain was isolated from the cecal content of a mouse; other members were isolated from the intestinal tract of humans. DNA G + C content (mol%) of Intestinimonas butyriciproducens : 58.4 (based on HPLC) and 59.4 (based on genome sequence). Type species : Intestinimonas butyriciproducens SRB‐521 T . Kläring, Hanske, Bui, Charrier, Blaut, Haller, et al. 2013, 4611 VP .