Introduction Adherence to control measures and provision of appropriate information at international borders and points of entry (POE) are key to limiting the importation of COVID-19. This study aimed to describe the knowledge, attitudes, and practices (KAPs) of POE staff towards COVID-19 in Botswana. Methods This was a cross-sectional study of the COVID-19 KAPs among workers at Tlokweng border and Sir Seretse Khama International Airport (SSKIA) using a self-administered questionnaire. The tool incorporated the participants’ demographics and selected questions on COVID-19 KAPs. Analysis was descriptive. Categorical data were summarized with frequencies while numeric data were summarized with medians and interquartile ranges (IQR). The total knowledge and practice scores of each individual were computed by adding their individual scores for each question. The scores were then categorized according to Bloom’s cutoffs of good (80–100%), moderate (60–79%) and poor (<60%). Results A total of 276 individuals participated in the study. Of these, 70 were from Tlokweng border and 206 were from SSKIA. The participants performed worst on questions on the frequency of severe disease and asymptomatic transmission of COVID-19. The attitudes were mainly positive. However, 54.6% of participants thought that the COVID-19 burden is exaggerated. For practice, the worst performance was on social distancing, sanitizing shared surfaces, and going to work while symptomatic. Overall, good and moderate knowledge was observed in 47.8% and 38.0% of participants, respectively. Similarly, good and moderate performance on practices was observed in 63.6% and 24.4% of participants respectively. Conclusion The knowledge, attitudes, and practices were generally good at the 2 points of entry. More than 85% of respondents had moderate or good performance on knowledge and practice questions. However, the respondents performed poorly in some key questions. Targeted health information and promotion must address the identified gaps.
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