Diabetic nephropathy is a major cause of renal tissue damage and complications of diabetes. In this in‐vitro experimental study, diabetes was induced by a single injection of Streptozotocin (55 mg/kg) in rats. STZ‐diabetic rats were treated with oral doses of methanolic extract T. ammi seeds (300–400 mg/kg). After 8 weeks, serum levels of glucose, creatinine, urea, SOD, and MDA in the blood serum and protein levels in urine were measured. Also, changes in the expression of IL‐18 and TNF‐α genes in the renal tissue were examined. The results of this study showed that T. ammi seeds have anti‐hyperglycemic and anti‐hyperlipidemia effects. It can also increase SOD, decrease MDA, and inhibit IL‐18 and TNF‐α expression. T. ammi methanolic extract reduced blood glucose, oxidative stress, renal function biomarkers, and inflammatory cytokines caused by STZ in mice. Because of this, it could be used as a new treatment for diabetic nephropathy.
Novelty impact statement
The effect of T. ammi seed extract on rats with nephropathy was investigated.
The real‐time PCR technique was used to measure the expression of the genes IL‐18 and TNF‐a, while the ELISA technique was used to measure the level of MDA and SOD serum.
T. ammi is a medicinal plant with anti‐inflammatory and anti‐nephropathy effects.
The objective of this study was to determine the effect of Trachyspermum ammi (T. ammi) on the liver of streptozotocin (STZ)-induced diabetic rats. This study began by identifying the methanolic composition of T. ammi. The presence of three compounds was determined using gas chromatography-mass spectrometry (GC-MS), with Thymol (60.705%), ϒ-Terpinene (22.216%), and P-cymene (17.078%) being the most prevalent. Afterwards, diabetic rats were treated with T. ammi (200–500 mg/kg) or losartan (20 mg/kg) daily for 60 days. In the diabetic rats treated with T. ammi, levels of hepatic indicators, lipid peroxidation markers, and proinflammatory mediators decreased significantly. In the liver of T. ammi-treated diabetic rats, glutathione-S-transferase (GST), catalase (CAT), and glutathione (GSH) increased significantly. Despite the fact that proinflammatory cytokine levels were significantly increased, interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels decreased in the groups administered the extract. From these results, it can be concluded that T. ammi significantly restored the liver’s antioxidant balance and inflammation caused by hyperglycemia.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.