Background and Aims: CD71 + erythroid cells are enriched during pregnancy with immuno suppressive properties. We investigated the frequency and functionality of CD71 + erythroid cells in peripheral blood, cord blood, and placenta of inflammatory bowel disease [IBD] patients versus healthy controls [HCs]. We aimed to determine their role in IBD pathogenesis during pregnancy. Methods: Peripheral blood was collected at preconception, the first, second and third trimesters, and postpartum. Cord blood and placental tissues were collected at the time of birth. Cells from different specimens were subjected to immunephenotyping and functional assays. CD71 + erythroid cells were purified for quantitative polymerase chain reaction [qPCR] analysis. Using an allogeneic mouse model of pregnancy, the effects of CD71 + erythroid cells depletion on intestinal homeostasis and dysbiosis was studied. Results: IBD patients had lower CD71 + erythroid cells during pregnancy compared with HCs. Placenta and cord blood CD71 + erythroid cells from IBD patients exhibited impaired functionality and expressed lower inhibitory molecules including VISTA, TGFβ, and reactive oxygen species [ROS]. Lower CD71 + erythroid cells were correlated with reduced regulatory T cells and increased immuneactivation in IBD patients. Depletion of CD71 + erythroid cells in an allogeneic pregnancy model resulted in upregulation of TLRs, IL6, and CXCL1, and enhanced production of TNFα, in intestinal tissues. In contrast, TGF β gene expression was reduced. Excessive inflammatory response in the gut [e.g. TNFα] affects intestinal integrity and CD71 + erythroid cells impact on the gut's bacterial composition. Conclusions: Reduced frequency and/or impaired functionality of CD71 + erythroid cells during pregnancy may predispose IBD patients to a more proinflammatory milieu in their gastrointestinal tract, characterised by lower Tregs, higher IL6, and TNFα, and dysbiosis.
We isolated Crithidia spp. from clinical samples of patients suspected of cutaneous leishmaniasis in Iran, indicating that Crithidia spp. are capable of surviving at human body temperature and infecting macrophage cells. This raises questions on the influence of this phenomenon on pathogenicity, therapeutic outcome, and disease control strategies. This article is protected by copyright. All rights reserved.
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