Objective: This review summarizes the methods of chitosan production as well as its various potential applications. Materials and Methods:This study has been performed by literature review. Results:Chitin is a natural compound that is the second most abundant biological compound in nature. Chitin is found in many fungi species and aquatic crustaceans like crabs, shrimps, and many insects. Shrimp is the source of one of the most rapidly increasing businesses in the world. However, during shrimp processing, the hard exoskeleton of shrimps, like shrimp skin and head portions, is discarded as bio-waste. This exoskeleton of shrimp contains a considerable amount of chitin. Chitosan is a bio-product that is produced from chitin by the deacetylation process. Either chemical or biological processes can carry out the deacetylation process. The massive number of chitin treated as bio-waste can be used to produce chitosan. Chitosan is a biocompatible compound, naturally biodegradable, and non-toxic, and this compound can be used in various applications. Chitosan has potential antimicrobial and antioxidant activities. Moreover, it can also be used in drug delivery, biotechnology, bionanotechnology, food technology, regenerative medicine, medicine, numerous industrial applications, gene therapy, cancer therapy, agriculture, environmental protection, and so on. Conclusion:Chitosan can be used in almost all fields of biology. Although chitosan is not still used in all the mentioned fields shortly, chitosan should significantly impact these areas. More researches should be performed to make chitosan a compound of many applications and possibilities.
Cellulase is a biocatalyst that hydrolyzes cellulosic biomass and is considered a major group of industrial enzymes for its applications. Extensive work has been done on microbial cellulase but fungi are considered a novel strain for their maximum cellulase production. Production cost and novel microbial strains are major challenges for its improvement where cheap agro wastes can be essential sources of cellulose as substrates. The researcher searches for more cellulolytic microbes from natural sources but the production level of isolated strains is comparatively low. So genetic modification or mutation can be employed for large-scale cellulase production before optimization. After genetic modification than in silico molecular modeling can be evaluated for substrate molecule’s binding affinity. In this review, we focus not only on the conventional methods of cellulase production but also on modern biotechnological approaches applied to cellulase production by a sequential study on common cellulase-producing microbes, modified microbes, culture media, carbon sources, substrate pretreatment process, and the importance of optimum pH and temperature on fermentation. In this review, we also compare different cellulase activity determination methods. As a result, this review provides insights into the interrelationship between the characteristics of optimizing different culture conditions, genetic modification, and in silico enzyme modeling for the production of cellulase enzymes, which may aid in the advancement of large-scale integrated enzyme manufacturing of substrate-specific enzymes.
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