To prevent the formation of biofilm on material surface, the latter must have antibacterial properties. The aim of this study is to investigate the synthesis and the antibacterial effect of a new N-halamine coating based on polydopamine (PDA). The interests of this coating are multiple, notably the green process used to prepare it and the wide variety of organic or inorganic materials that can be functionalized. First the formation of the PDA coating by oxidative polymerization of dopamine in weak alkaline aqueous solution was studied and characterized. Then, these PDA films were exposed to a NaOCl solution in order to form chloramine functions into the coating, i.e. to immobilize oxidative chlorine on and into the coating. The PDA film chlorination was notably followed in situ by quartz crystal microbalance (QCM). The influence of the NaOCl solution pH and concentration on chlorination kinetics and on PDA film degradation was evidenced. Finally, the antibacterial properties of the modified PDA coatings were highlighted by testing their antiadhesion and bactericidal properties towards Escherichia coli bacterial strain.
Due to the ability of microorganisms to first adhere to a material surface and then to lead to the formation of a biofilm, it is essential to develop surfaces that have antimicrobial properties. It is well known that N-halamine coatings allow us to prevent or minimize such phenomena. In the present work, various polydopamine (PDA) coatings containing chloramine functions were studied. In fact, three PDA-based films were formed by the simple immersion of a gold substrate in a dopamine solution, either at pH 8 in the presence or not of polyethyleneimine (PEI), or at pH 5 in the presence of periodate as an oxidant. These films were characterized by polarization modulation reflection absorption infrared spectroscopy and X-ray photoelectron spectroscopy analyses, and by scanning electron microscopy observations. The chlorination of these PDA films was performed by their immersion in a sodium hypochlorite aqueous solution, in order to immobilize Cl(+I) into the (co)polymers (PDA or PDA–PEI). Finally, antibacterial assays towards the Gram-negative bacteria Escherichia coli (E. coli) and the Gram-positive bacteria Staphylococcus epidermidis (S. epidermidis) were conducted to compare the bactericidal properties of these three N-halamine coatings. Regardless of the bacteria tested, the PDA coating with the best antibacterial properties is the coating obtained using periodate.
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