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Abstract:The fungicidal and nematicidal activity of an emulsifiable formulation of furfural [2-furancarboxaldehyde] against root rot and root-knot pathogens was studied in laboratory, greenhouse and field experiments. The linear growth of tested soilborne pathogenic fungi was dramatically reduced with the increasing of furfural concentrations added to the growth medium up to 4 000 ppm where no growth was observed, while the bacterial and fungal bioagents showed more tolerance to these concentrations and failed to grow at 6 000 and 7 000 ppm, respectively. Pot and field experiments indicated that furfural at 6 000 ppm combined with bioagent treatments proved to have superior suppressive effect against tomato root rot incidence, caused by Fusarium solani and Rhizoctonia solani, comparing with each individual treatment. Numbers of nematodes in soil declined sharply in direct response to furfural application with the sharpest reductions in its population. No symptoms of root-knot incidence, caused by Meloidogyne incognita as well as no detected galls and eggmasess were observed in the root system of tomato plants grown in either artificially or naturally infested soil with the parasite at the same concentration under greenhouse and field conditions. Results from these experiments indicate that a variety of effective broad-spectrum formulations of furfural can be developed for control of economically important soilborne pests.

Control measures of postharvest diseases of strawberry and navel orange fruits using hydrogen peroxide, calcium chloride and chitosan were evaluated under in vitro and in vivo conditions. All tested concentrations of chemicals used were able to reduce the linear growth and spore germination of B. cinerea; R. stolonifer; P. digitatum and P. italicum. Complete inhibition of linear growth and spore germination was obtained with concentrations of 1.5 and 2.0% of all treatments. Under storage conditions, significant reduction in descending order of mould incidence was observed in strawberry and orange fruits treated with ascending concentrations of calcium chloride, hydrogen peroxide and chitosan. Obtained data revealed significant reduction in mould incidence in fruits when treated by calcium chloride and chitosan 12h before artificial inoculation with the mould pathogens, while hydrogen peroxide showed the opposite result. The present study demonstrated that the application of hydrogen peroxide is superior to treatment with calcium chloride or chitosan enhanced the control activity against mould pathogens which as it expressed was as either percentage of diseased fruits or decay development as rotted tissue weight of strawberry and navel orange. The applied tested chemical might act as contact and systemic fungicides which have a protective or therapeutic effect.

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Maize (Zea mays L.) is one of the most economic crops in Egypt. Production of amylase from the waste of maize is the most economic and cheap renewable and most abundant raw materials present in environment. Biosynthesis of Cu-chitosan nanoparticles for amylase production by co-culturing between Trichoderma harzianum and Pseudomonas fluorescens at different ratios compared to free conditions was the main purpose of this study. The optimum ratio 8:2, recorded between P. fluorescens: T. harzianum, was the most promising for production of amylase produce 22.47 and 28.60 U/ml for free and nano, respectively. The UV visible spectral analysis Cu-chitosan NPs was 220 nm, while the mean diameter, using transmission electron microscopy was 0.5 μm. Application of fermented maize wastes by co-cultivation of P. fluorescence and T. harzianum, as a grain dressing before sowing declared the reduction in both root and foliar diseases during the maize growing season, starting from germination up to 70 days of its vegetative growth under field conditions. A promising approach is the creation and use of environmentally safe products, whose protective effect is based on the induction of hydrolase inhibitors in plants.

Background Stem rust caused by Puccinia triticina Erikss is one of the most devastating fungal foliar diseases, and causes substantial yield losses to wheat crops cultivated under field conditions. This study aimed to evaluate the efficacy of plant extract-based foliar sprays for wheat with carnation, ginger and cinnamon on the severity of stem rust infection of three wheat cultivars cultivated under field conditions for two consecutive winter seasons of (2020/21 and 2021/22). Results In both seasons our data showed that all treatments were associated with some degree of disease suppression recorded as a range of 2.3–8.6% compared to 15.3–24.5% in untreated control treatment. The plant extract treatment was more effective than Amistar, a commercial fungicide. They recorded disease severity as: scores ranged between 2.3 and 3.2%; 3.0–4.5%, and 3.0–6% for wheat cvs. Giza 1, Misr 2, and Sids 14, in respective order. Meanwhile, scores of 4.1, 7.5, and 8.6% were recorded at fungicidal treatment. Spaying wheat plants with carnation extract showed the lowest disease prevalence followed by ginger and cinnamon extract, respectively. Increased in grain yield was associated with reduced disease severity. Conclusions The present investigation demonstrated the efficacy using plant extracts against foliar diseases caused by Puccinia and it was suggested that they may be an alternative to traditional chemical treatment.

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