Objectives:We aim to address public knowledge, attitudes, and practices relative to prenatal genetic testing as a starting point for policy development in Jordan.Study design:We conducted a cross-sectional prenatal genetic testing knowledge, attitudes, and practices survey with 1111 women recruited at obstetrics and gynecology clinics nationwide. Data were analyzed using a variety of descriptive and inferential statistical tests.Results:The overwhelming majority (>94%) of participants considered prenatal genetic testing, particularly non-invasive prenatal genetic screening, procedures to be good, comfortable, and reasonable, even when the non-diagnostic nature of non-invasive prenatal genetic screening was explained. Likewise, 95% encouraged the implementation of non-invasive prenatal genetic screening within the Jordanian health system, but most preferred it to remain optional. However, women in higher-risk age brackets, in consanguineous marriages, and with less education were significantly less interested in learning about non-invasive prenatal genetic screening. Only 60% of women interviewed were satisfied with the services provided by their obstetric/gynecologist. The more satisfied the women were, the more they are likely to adapt non-invasive prenatal genetic screening.Conclusions:In sum, although the data support the receptivity of Jordanian women to national implementation of non-invasive prenatal genetic screening, such policies should be accompanied by health education to increase the genetic literacy of the population and to engage high-risk populations. Thus, this offers rare insight into the readiness of 1 particular Arab population to adapt non-invasive prenatal genetic screening technologies.
A simple and fast method for the determination of PAHs in smoked meat samples was described. The QuEChERS (Z-Sep) procedure was used for sample preparation. Gas chromatograph-mass spectrometer with electron ionization (EI) was used to separate and detect the PAHs. All 16 common PAHs were analyzed successfully. Matrix-matched calibration was applied. Spiked samples were performed at 1 ng/g (n=10) and 10 ng/g (n=10) for two days. Overall recoveries of PAHs were within 74 to 117%, with RSDs within 1.15 to 37.57% and 1 and 10 ng/g wet weight for first and second day, respectively. In most of the analyzed smoked meat samples, there were no exceeded levels compared to the maximum levels declared by Commission Regulation (EU) number 835/2011. The method can be recommended for routine analysis for laboratories having a large number of samples.
Murad Helaleh1, Ilhame Diboun2, Nada Altamimi1, Aishah Latiff1, Mohamed Elrayess1* 1Toxicology and Multipurpose Lab, Anti Doping Laboratory Qatar, Sports City, Doha, Qatar. 2 Department of Economics, Mathematics and Statistics, Birkbeck, University of London, London WC1E 7HX, UK. Corresponding author: melrayess@adlqatar.qaAbstractPolybrominated diphenyl ethers (PBDEs) represent a class of widely utilized flame retardants [1]. With over 200 congeners that vary by the extent of halogenations, various PBDEs can leak freely into the environment [2, 3]. Despite cessation of their manufacturing, concerns of their bioaccumulation remain [4-6] due to their stability in products manufactured before the ban and recycled materials [1]. With their high lipophilicity, PBDEs tend to accumulate in adipose tissue, potentially altering the function of this endocrine organ by increasing lipolysis and decreasing glucose oxidation, causing increased risk of metabolic disease including obesity, insulin resistance and type 2 diabetes [7]. Exposure to PBDE-47, for example, during the early post-natal period was shown to induce disturbance in glucose metabolism causing insulin resistance in susceptible mice [8]. In this study, levels of various PBDEs were assessed in subcutaneous and omental adipose tissues from 33 obese and morbidly obese patients (11 insulin sensitive and 22 insulin resistant) and their correlation with mediators of metabolic disease were established. Our results suggested that out of 22 detectable PBDEs in subcutaneous and omental adipose tissues, PBDE99, 28, 47 and 126 were significantly higher in insulin resistant individuals compared to their insulin sensitive counterparts. When considering PBDEs congeners, penta congeners as a group were also higher in insulin resistant individuals compared to insulin sensitive counterparts, while no significant differences were detected in mono, tri, tertra, hexa, hepta and octa congeners between the two studied groups. This data suggest that accumulation of various PBDEs in human adipose tissues obtained from obese individuals is associated with increased risk of insulin resistance. Further investigation of the functional relevance of these associations is currently underway. This study is partly funded by QNRF's grant number NPRP6-235-1-048. References 1. Birnbaum, L.S. and D.F. Staskal, Brominated flame retardants: cause for concern? Environ Health Perspect, 2004. 112(1): p. 9-17. 2. Chen, D. and R.C. Hale, A global review of polybrominated diphenyl ether flame retardant contamination in birds. Environ Int, 2010. 36(7): p. 800-11. 3. Ernest, S.R., et al., Effects of chronic exposure to an environmentally relevant mixture of brominated flame retardants on the reproductive and thyroid system in adult male rats. Toxicol Sci, 2012. 127(2): p. 496-507. 4. Kelly, B.C., et al., Bioaccumulation behaviour of polybrominated diphenyl ethers (PBDEs) in a Canadian Arctic marine food web. Sci Total Environ, 2008. 401(1-3): p. 60-72. 5. Mercado-Feliciano, M. and R.M. Bigsby, Hydroxylated metabolites of the polybrominated diphenyl ether mixture DE-71 are weak estrogen receptor-alpha ligands. Environ Health Perspect, 2008. 116(10): p. 1315-21. 6. Sjodin, A., D.G. Patterson, Jr., and A. Bergman, A review on human exposure to brominated flame retardants–particularly polybrominated diphenyl ethers. Environ Int, 2003. 29(6): p. 829-39. 7. Hoppe, A.A. and G.B. Carey, Polybrominated diphenyl ethers as endocrine disruptors of adipocyte metabolism. Obesity (Silver Spring), 2007. 15(12): p. 2942-50. 8. McIntyre, R.L., et al., Polybrominated diphenyl ether congener, BDE-47, impairs insulin sensitivity in mice with liver-specific Pten deficiency. BMC Obes, 2015. 2: p. 3.
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