As water deficit in arid countries has already become the norm rather than the exception, water conservation in crop production processes has become very critical. Therefore, it is urgent to develop feasible strategies to achieve this goal. Exogenous application of salicylic acid (SA) has been proposed as one of the effective and economical strategies for mitigating water deficit in plants. However, the recommendations concerning the proper application methods (AMs) and the optimal concentrations (Cons) of SA under field conditions seem contradictory. Here, a two-year field study was conducted to compare the effects of twelve combinations of AMs and Cons on the vegetative growth, physiological parameters, yield, and irrigation water use efficiency (IWUE) of wheat under full (FL) and limited (LM) irrigation regimes. These combinations included seed soaking in purified water (S0), 0.5 mM SA (S1), and 1.0 mM SA (S2); foliar spray of SA at concentrations of 1.0 mM (F1), 2.0 mM (F2), and 3.0 mM (F3); and combinations of S1 and S2 with F1 (S1F1 and S2F1), F2 (S1F2 and S2F2), and F3 (S1F3 and S2F3). The results showed that the LM regime caused a significant reduction in all vegetative growth, physiological, and yield parameters, while it led to an increase in IWUE. The application of SA through seed soaking, foliar application, and a combination of both methods increased all of the studied parameters in all the evaluated times, resulting in higher values for all parameters than the treatment without SA (S0). The multivariate analyses, including principal component analysis and heatmapping, identified the foliar application method with 1–3 mM SA alone or in combination with seed soaking with 0.5 mM SA as the best treatments for the optimal performance of wheat under both irrigation regimes. Overall, our results indicated that exogenous application of SA has the potential to greatly improve growth, yield, and IWUE under limited water application, while optimal coupling combinations of AMs and Cons were required for positive effects in field conditions.
Sequence-related amplified polymorphism (SRAP) markers were used to assess the genetic diversity among a collection of 52 sesame accessions representing different geographical environments, including eight Saudi landraces. A combination of seventeen primers generated a high number of alleles (365) with 100% polymorphism. The polymorphic information content (PIC) and primer discrimination power (DP) recorded overall means of 0.88 and 5.88, respectively. Genetic similarity values based on Jaccard coefficients ranged from 0.12 to 0.49, with an average similarity value of 0.30, indicating both high genetic distance and a wide genetic basis of the investigated accessions. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram grouped 48 of 52 accessions into seven main clusters, and five accessions failed to form clusters and were separated individually. However, subclusters separated the accessions and, considering the relatedness of accessions and their geographical origin, formed distinct diversity among groups. Saudi landraces showed the widest genetic basis compared with other introduced accessions that were distributed throughout the dendrogram, indicating that agro-ecological zones were indistinguishable by cluster analysis. SRAP analysis revealed a high degree of genetic polymorphism in sesame accessions investigated and showed weak association between geographical origin and SRAP patterns. This wide genetic variability should be considered for sesame breeding programs.
Innovations in water-saving cultivation strategies are urgently needed to achieve high yield and elevated water use efficiency (WUE) simultaneously in arid regions with limited water resources. Here, we conducted a two-year field study to compare the impacts of eight combinations of planting patterns (PPs) and mulching on the soil water content (SWC) in the top 60 cm soil layer, the growth, the yield, and the WUE of wheat under two irrigation rates (1.00 and 0.50 ET). These combinations included three conventional flat planting (CF) patterns, including CF without mulch (CFNM), with plastic film (CFPM), and with wheat straw mulch (CFSM); three raised-bed planting (RB) patterns, including RB without mulch (RBNM), with plastic film (RBPM), and wheat straw (RBSM) mulch; and two ridge–furrow planting (RF) patterns, including RF without mulch (RFNM) and with plastic film mulch (RFPM). The results showed that the tested treatments affected the SWC at different depths under both irrigation rates. Compared with the two non-mulched treatments under 0.50 ET, the SWC of the three PPs with plastic film and the two PPs with wheat straw mulching were significantly higher before irrigation by 14.4–22.0% and 6.9–17.2% at 0–20 cm soil depth, 16.4–29.0% and 6.6–14.9% at 20–40 cm soil depth, and 3.3–34.8% and 3.4–14.5% at 40–60 cm soil depth, respectively. All measured wheat parameters, except harvest index, were significantly affected by the interaction between irrigation rate and PPs. The highest values for plant dry weight (PDW), yield components, grain yield (GY), and WUE under 1.00 ET were obtained in the two PPs with wheat straw mulch, while the three PPs with plastic film showed the highest values of these parameters under 0.50 ET. The yield response factor (Ky) based on PDW was acceptable for all PPs mulched with plastic film and wheat straw as well as for RFNM, while Ky based on GY was acceptable only for the PPs mulched with plastic film and for RFNM, as the Ky values of these PPs were less than 1 under 0.50 ET. The SWC at different depths exhibited quadratic and nonsignificant relationships with all parameters under 1.00 ET, while these relationships were linear and strong under 0.50 ET, with a few exceptions. Overall, we conclude that combining any PPs with plastic film mulching could be used as a feasible and effective strategy for obtaining high wheat yield and WUE in the irrigated and arid agroecosystem.
Successfully evaluating and improving the salt tolerance of genotypes requires an appropriate analysis tool to allow simultaneous analysis of multiple traits and to facilitate the ranking of genotypes across different growth stages and salinity levels. In this study, we evaluate the salt tolerance of 56 recombinant inbred lines (RILs) in the presence of salt-tolerant and salt-sensitive control genotypes using multivariate analysis of plant dry weight, measured at 75 (PDW-75) and 90 (PDW-90) days from sowing, biological yield (BY), grain yield (GY), and their salt tolerance indices (STIs). All RILs and genotypes were evaluated under the control and 15 dS m−1 for two consecutive years (2019/2020 and 2020/2021). Results showed significant main effects of salinity and genotype as well as their interactions on four plant traits. Significant genotypic differences were also found for all calculated STIs. STIs exhibited moderate to strong relationships with the four plant traits when measured under either the control or salinity conditions and between each other. The principal component analysis (PCA) showed that the most variation among all analyzed variables was explained by the first two PCs, with the PC1 and PC2 explained at 61.8–71.8% and at 28.0–38.2% of the total variation, respectively. The PC1 had positive and strong correlations with the four plant traits measured under salinity conditions and STI, YI, REI, SWPI, MRPI, MPI, GMPI, and HMPI. The PC2 had strong correlations with BY and GY measured under the control conditions and SSI, TOL, RSE, and YSI. The PC1 was able to identify the salt-tolerant genotypes, while the PC2 was able to isolate the salt-sensitive ones. Cluster analysis based on multiple traits organized 64 genotypes into four groups varied from salt-tolerant to salt-sensitive genotypes, with the salt-tolerant group attaining higher value for plant traits under salinity conditions and the STIs related to the PC1. In conclusion, the use of multivariate analysis together with the STIs that evaluated the performance of genotypes under contrasting environmental conditions will help breeders to distinguish salt-tolerant genotypes from salt-sensitive ones, even at the early growth stages of plant development.
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