This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, Syngnathus abaster, Uroteuthis (Photololigo) chinensis, Verticillium dahliae, Wasmannia auropunctata, and Zygochlamys patagonica. These loci were cross-tested on the following species: Chaetodon baronessa, Falco columbarius, Falco eleonorae, Falco naumanni, Falco peregrinus, Falco subbuteo, Didelphis aurita, Gracilinanus microtarsus, Marmosops paulensis, Monodelphis Americana, Odontesthes hatcheri, Podocarpus grayi, Podocarpus lawrencei, Podocarpus smithii, Portunus pelagicus, Syngnathus acus, Syngnathus typhle,Uroteuthis (Photololigo) edulis, Uroteuthis (Photololigo) duvauceli and Verticillium albo-atrum. This article also documents the addition of nine sequencing primer pairs and sixteen allele specific primers or probes for Oncorhynchus mykiss and Oncorhynchus tshawytscha; these primers and assays were cross-tested in both species.

Ferroptosis constitutes a novel type of cell death in morphology, biochemistry and genetics, and is tightly associated with the occurrence, development, prognosis and treatment of tumors. Nevertheless, the potential contribution of ferroptosis-related genes (FRGs) to the tumor microenvironment (TME) is indistinct. We delineated and evaluated the expression pattern of FRGs in gastric cancer (GC) samples from the perspectives of genetics and transcription. Subsequently, we discerned two different molecular subtypes and figured out that the changes in multilayer FRGs are linked with the clinicopathological characteristics, prognosis and TME cell infiltration characteristics of patients. Then, we constructed an FRG-score with a view to predicting overall survival (OS) and verified its predictive ability in GC patients. Therefore, we constructed a high-precision nomogram to improve the clinical applicability of the FRG-score. Low FRG-score, due to its high microsatellite instability (MSI-H), high mutational load and immune activation, indicates the possible advantage of OS. In addition, the FRG-score was closely related to the cancer stem cell (CSC) index and the sensitive degree of chemotherapeutic drug. Our generalized study of FRGs in gastric cancer shows their potentialities in the TME, clinicopathological characteristics and prognosis. These results may advance the existing knowledge of FRGs in gastric cancer, and push ahead with more effective prognostic assessment and the development of more effective immunotherapy approaches.

Purpose: Ferroptosis is a new type of cell death in morphology, biochemistry and genetics, and is closely related to the occurrence, development, prognosis and treatment of tumors. However, the potential role of ferroptosis-related genes (FRGs) in the tumor microenvironment (TME) is indistinct. Methods: We described and evaluated the expression pattern of FRGs in gastric cancer (GC) samples from the fields of genetics and transcription. Subsequently, we identified two different molecular subtypes and found the changes in multilayer FRGs. Then, we constructed an FRG-score to predict overall survival (OS) and verified its predictive ability in GC patients. Therefore, we constructed a high-precision nomogram to improve the clinical applicability of the FRG-score. Results: The changes in multilayer FRGs are related to the clinicopathological characteristics, prognosis and TME cell infiltration characteristics of patients. Low FRG-score, due to its high microsatellite instability (MSI-H), high mutation burden and immune activation, indicates the possible advantage of OS. In addition, the FRG-score was closely correlated with the cancer stem cell (CSC) index and chemotherapeutic drug sensitivity. Conclusions: Our generalized analysis of FRGs in gastric cancer shows their potential role in the TME, clinicopathological characteristics and prognosis.