Siunmary:A method based on high performance liquid chromatography with electrochemical detection is described for quantification of adrenaline, noradrenaline, dopamine (free and total), metanephrine, normetanephrine, and 3-methoxytyramine (free) in urine samples. Purification of the samples is achieved on disposable -Rad ion-exchange columns in combination with small Sephadex G 10 columns. A clear circadian rhythm was found in the 24 h excretion pattern of free adrenaline and noradrenaline for 4 volunteers (city bus drivers during a working day). No rhythms were seen in the 24h excretion pattern of free or conjugated dopamine, conjugated adrenaline or conjugated noradrenaline, or of the methylated free catecholamines. It is concluded that in order to obtain Information about the neurotransmitter function of peripheral catecholamines it is sufficient to determine the non-conjugated compounds; values for the conjugated and methylated metabolites contribute no extra Information. 24-Stunden^-Ausscheidungsmuster der freien, konjugierten und methylierten Katecholamine beim MenschenZusammenfassung: Eine Methode zur quantitativen Bestimmung von Adrenalin, Noradrenalin, Dopamin (freies und gesamtes), Metajjephrin, Normetanephrin und 3-Methoxytyramin (freies) im Harn auf der Grundlage der Hochleistungsflüssigchromatographie mit elektrochemischer Detektion wird beschrieben. Die Reinigung der Proben wird durch Einmäl-Ionenaustauscher-Säulen ( -Rad) in Kombination mit kleinen Sephadex G -Säulen erreicht. Bei vier Busfahrern wurde während eines Arbeitstages ein deutlicher circadianer Rhythmus im 24^Stunden*Ausscheidungsmuster für freies Adrenalin und Noradrenalin gefunden. Für freies öder konjugiertes Dopamin, konjugiertes Adrenalin, konjugiertes Noradrenalin oder die methylierten freien Katecholamine wurde keine Rhythmik im 24-Stunden-Ausscheidungsmuster beobachtet. Wir schließen daraus, daß es für den Erhalt von Informationen über die Neurotransmitterfunktion der peripheren Katecholamine ausreicht, die nicht-konjügierten Verbindungen zu bestimmen; Werte für die konjugierten und methylierten Metabolite erbringen keine weitere Information.is some recent evidence that also methylated catecholIntroduetion amines should be taken into account äs indices of Psychoendocrine research has emphasized the use of sympathieoadrenal activity (5). It is believed that Ocätecholamines äs indicators of mental stress and methylation of catecholamines by the extraneuronally physical workload (l, 2). Adrenaline (A) excretion localized enzyme, catechol-O-methyl transferase, is appears to be particulary responsive to mental stress, the primary metabolic route for actively released catewhereas urinary noradrenaline responds mainly to cholamine. This enzyme converts noradrenaline, adphysical activity (3, 4). Although studies in this field renaline and dopamine into nonnetanephrine, metaare usually restricted to the free catecholamines, there nephrine, and 3-methoxytyramine, respectively.
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