The identification of quantitative trait loci (QTL) across different environments is a prerequisite for marker‐assisted selection (MAS) in crop improvement programmes. CottonSNP63k Illumina infinium array was used for genotyping 178 inter‐specific recombinant inbred lines and the parents, and identified 1,667 homozygous polymorphic markers between the parents. Of these, 1,430 markers were used for the construction of linkage map after removing 237 redundant markers. The genetic map spans a total genetic length of 3,149.8 cM with an average marker interval size of 2.2 cM. The phenotypic data from five environments were analysed separately using inclusive composite interval mapping which identified a total of 56 QTL explaining phenotypic variances (PVE) in the range of 8.18%–28.91%. There were 11 and 24 major QTL found for fibre quality and yield components, respectively. A total of 64 QTL were identified through Multi‐Environment Trials analysis, of which 34 recorded QTL × Environment interactions.
Chickpea (Cicer arietinum L.) is one of the most cultivating pulse crops globally and in India, its productivity is limited blisteringly by Gram pod borer insect (Helicoverpa armigera) and Fusarium wilt disease. Pyramiding of these two biotic stress resistance in a single genotype is expected to increase crop productivity and reduces the usage of pesticides/fungicides by farmers, which boost economic viability in the cultivation of this crop. Hence, we planned to transfer the cry1Ac gene, which imparts pod borer resistance from BS 100B event, to Super Annigeri-1(SA-1), the wilt resistant variety bred through marker-assisted backcrossing.The F1 plants developed by SA-1 X BS 100B crosses were confirmed with cry1Ac gene-specific marker and polymorphic SSR marker (ICCM0299).The expression of the cry1Ac gene at the transcriptional level through reverse transcription polymerase chain reaction and at the protein level through enzyme-linked immuno sorbent assay was confirmed in F1. The presence of a single copy of a gene integration, stably, was confirmed through the inheritance of the cry1Ac gene and 3:1 segregation ratio in F2 using Chi-square test. BS 100B (donor parent), F1 and F2 plants, respectively recorded 21.47 µg, 20.43 µg and 15.31-21.17µg of Cry1Ac protein/g of leaf tissue in quantitative ELISA test that is enough to record pest resistance. This is the first study to combine resistance to both pod-borer and Fusarium wilt by intercrossing of one cry1Acevent (BS 100B) together with SA-1 developed through molecular breeding and developed progenies have shown resistance to both biotic stresses.
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