Milk chocolate is particularly popular with different age groups. It is characterized by low protein content with a large amount of fats and carbohydrates determining the food imbalance of the product. In conditions of high-grade animal proteins deficiency the selection of high-quality protein-containing ingredients for food production is very relevant. The aim of this work is to study the protein adequacy of various components of milk chocolate to enhance its biological value. The amino acid scale method has been used to assess the biological value of proteins; it is based on the determination of amino acid (chemical) score. It has been found that the limiting biological value amino acid for classical white raw ingredients of milk chocolate (cocoa products and milk powder) is methionine + cysteine. For constructing chocolate formulas with increased biological value it is advisable to use protein-containing raw materials (whey protein concentrate, oat flour, etc.) to compensate for the limiting amino acids. The indicator of amino acids utilitarianity of proteins of raw milk chocolate components has been calculated. On the basis of the utilitarian index we have established the coefficient of utilitarian of the amino acid composition of the raw materials characterizing essential amino acids' balance. We have determined the biological value of protein and the amino acid composition imbalance coefficient. It has been found that the amino acid composition of milk and whey protein concentrates is most balanced compared to the amino acid composition of traditional protein-containing raw components of milk chocolate. The limiting acid of whey protein concentrate is valine, which makes its use in the manufacture of chocolate products more attractive compared to milk protein concentrate (the limiting amino acid is methionine + cysteine). In the group of vegetable non-conventional raw materials oat and buckwheat flour are characterized by the best indicators of biological value. The amino acid adequacy of oat flour is comparable to the qualitative protein indicators of cocoa products, the limiting amino acid is lysine. Buckwheat flour is characterized by the smallest imbalance in amino acid composition, which distinguishes the proteins of this raw material with the highest degree of digestibility compared with the proteins of all the studied protein-containing components of milk chocolate.
Chocolate glaze consists mainly of sugar (50%) and fat (35%) and is a high-calorie semi-finished product with a high content of simple carbohydrates. The standard for glaze provides the possibility of producing chocolate glaze with fruit and vegetable components, which reduces the content of simple carbohydrates. Studied and justified the criteria for the production of chocolate glazes with different contents of fruit and vegetable powders. Analysis of the rheological parameters of chocolate semi-finished products showed that the introduction of fruit and vegetable powder instead of part of the sugar leads to a significant change in the nature of the rheological behavior of the semi-finished product. The yield strength increases from 3.4 to 12.6 Pa as the proportion of fruit and vegetable powder in the formulation increases. In order to determine the effect of the production method on changes in the yield strength of fruit and vegetable chocolate glaze, a series of experiments was carried out. It was found that with a constant content of fruit and vegetable powder (11%), temperature, processing time and machine support, the resulting chocolate glazes differed in their rheological characteristics. It was found that the greatest reduction in the yield strength of fruit and vegetable chocolate glazes during the production process can be achieved by stage-by-stage grinding of the formulation components in a ball mill and the additional introduction of PGPR emulsifier at the conching stage. The quality of chocolate semi-finished products depends not only on rheological parameters, but also on crystallization properties. The developed chocolate glazes with different contents of the fruit and vegetable component were processed at a temperature of about 30 °C in a laboratory tempering machine in order to form and stabilize fat crystals in ?-form. Then, the temperindex was determined. It was found that, subject to the same tempering conditions, with an increase in the proportion of fruit and vegetable powder, the temperature of glazes increases. Glazes with a mass fraction of fruit and vegetable powder of more than 7% were distinguished by the properties of re-tempered semi-finished products - the presence of large fat crystals, a rough structure and the absence of surface gloss. In order to obtain the optimal temperature index of glazes with a content of fruit and vegetable raw materials of more than 7%, their tempering modes were developed.
One of the main reasons of the confectionery product organoleptic parameters is microorganism activity. Growth most of them can be prevented or inhibited by the control of initial content. The influence of microorganisms with lypolityc activity to the cocoa-containing confectionery production quality was determined and the mathematic correlations were established, according which can be predicted changing of production characteristics on the basis of them initial content. Quantity of the mesophyll aerobic and optionally anaerobic microorganisms (QMA&OAM) were determined in accordance with GOST 33536-2015. Some microorganism colonies were separated by the thinning stroke inoculating on the solid nutrient agar. Testing cultures have been grown by the surface cultivation method on solid mediums and on liquid nutrient mediums (submerged cultivation). For the microorganisms studying was used microscopical method, biochemical characteristics were estimated by the standard methods. The conclusion about genus and species of the cultures was prepared on the basis of identification by mean the 16S rRNA analysis. The cocoa-containing product models were created which shown the organoleptic spoilage processes. For the basis was chosen mixture of the cocoa powder, sugar powder, and fats of the lauric types and four formulas A,B,C and D of the confectionery and coca-containing glazes analogues with minimum and maximum content of the total evaporated residue cocoa which is the regulated merit rate of the coca-containing confectionery products. Based upon the experimental results was determined dependence of the cocoa-containing confectionery products acidity change which can be used for prediction of them quality changes.
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