ÖZETAmaç: Bağırsak parazitlerinin tanısında direkt mikroskobik incelemenin ve farklı mikroskobistlerden elde edilen sonuçlar arasındaki farklılığın öneminin vurgulanması amaçlanmıştır. Yöntemler: Diyareli 225 çocuktan gaita örnekleri toplandı, makroskobik inceleme sonrası formalin-eter çöktürme yöntemiyle hazırlandı ve birbirinden bağımsız 3 farklı araştırmacı (parazitoloji uzmanı, mikrobiyoloji uzmanı ve ikinci yılını tamamlamış bir mikrobiyoloji araştırma görevlisi) tarafından mikroskobik incelemeye alındı.
It is known that infections caused by intestinal protozoa and helminths affect over 3.5 million people worldwide. In this case report, a patient with complaints of stomach ache for a long time who received thermal treatment is presented. During this thermal treatment, diarrhoea occurred and multiparasitism was diagnosed with two helminths; pseudoparasitism and multiprotozoa, simultaneously. Stool samples were collected from the patient on three consecutive days and one day after the treatment. All of the samples were prepared with formalin-ether sedimentation techniques after macroscopic and direct microscopic investigation. Cellophane-tape method for Enterobius vermicularis and Taenia spp. and Erlich-Ziehl-Neelsen staining method for coccidian parasites were used. At least four preparations were performed for each sample and serum physiologic, lugol' solution and trichrome stain were used for microscopic investigations.The motile segment she brought was investigated microscopically with Indian ink and identified as Taenia saginata. Under direct microscopy, Blastocystis hominis, Endolimax nana and Fasciola hepatica were seen. By formalin-ether sedimentation techniques, Ascaris lumbricoides, Fasciola hepatica, Blastocystis hominis, Endolimax nana and Entamoeba coli were identified. In recent years, intestinal parasitism is rarely seen in our city; therefore, multiparasitism in an adult and immunocompetent patient is interesting.
from two different pediatric emergency services, and randomly chosen 219 school children of 6 and 13 years of age from different socioeconomic environments and who did not have diarrhea. Stool samples were prepared with native-lugol and examined and then stained with trichrome and further examined under a light microscope.Genomic DNA was extracted from the stool samples using QIAamp® DNA Stool Mini Kit The extracted DNA samples were examined in terms of the presence of Blastocystis sp. using the realtime PCR method with the genesig® Standard Kit (Primer Design., UK) designed for the quantification of the Blastocystis G elongation factor-1 alpha gene. The PCR was performed using seven subtypespecific sequenced tagged site (STS) primers (SB83, SB155, SB227, SB332, SB340, SB336 and SB337) for the genotyping of Blastocystis sp. The collected data was analyzed using the SPSS (Version 17, Chicago IL, USA).Results: 115 samples were found positive for Blastocystis sp.. Subtyping was successfully performed on 46 samples using sequenced-tagged site (STS) primers and the PCR method. The most frequently detected subtype was ST3 (43.4%) followed by ST1 (26.1%), ST4 (10.9%) and ST2 (8.7%). The mixed subtypes were identified in five samples (10.9%) as; ST1+ST3 (n = 3), ST1+ST2 (n = 1) and ST2+ST3 (n = 1). None of the samples had ST5, ST6 or ST7. No statistically significant difference was found between the symptomatic and asymptomatic groups in terms of the Blastocystis sp. positivity and the distribution of subtypes (p > 0.05).
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