The global literature constantly receives new data showing the infectious pathogens as factors for development of atherosclerosis and acute cerebrovascular pathology, and the data showing the predictors of pathology of the heart-vessels as markers of inflammation. The results of research about the connection between the infectious agents and atherosclerosis are ambiguous, and the attempts to prove such connection have encouraged the experiments where the infectious agents with atherogenesis are modeled in animals. The connection of this data with ischemic lesion of brain is not properly explored, but certain experimental research show progressive degeneration in brain hemorrhage in mice with herpes virus 1 (HSV1) due to the post-stroke immunosuppression and reactivation of the infectious agent. The aim of this work is to explore experimentally the possible connection between the herpetic infection and the ischemic lesion to the cortex of mice. To achieve this goal we formed 5 groups of experimental animals (mice) for investigation into the possibility of connection between herpes infection and ischemiazaition of brain: 1st group (n=52) - with cholesterol diet; 2nd group (n=23) - with HSV1; 3d group (n=30) - with unilateral occlusion of the common carotid artery; 4th group (n=10) - with HSV1 and occlusion of the common carotid artery; 5th group (n=6) - with cholesterol diet, HSV1, occlusion of the common carotid artery. The microscopic slides evaluated changes morphometrically in the density of the neurons of the neocortex in the ocular of parietal temporal fields of the brain and the hippocampus. The results were processed statistically with Origin Lab 8.0. A probable increase in structural changes was identified in group 5 (with three pathological factors respectively) of the combined model compared to models without association and/or with several pathological signs from the groups 1-4 (1, 2, 3, 4). Comparison of the results between the 1-4th and 5th groups showed a significant increase in the relative number of neurons with cytopathological signs (hemochromatosis, deformation of the perikaryon, karyopyknosis), which may testify in favor of a sufficiently rapid lesion of the pyramidal neocortex neurons influenced by two or more pathological signs. The reduction in the density of pyramidal neurons in the temporal and/or temporal cortex in the combined model with ischemiazaition and viral association in was had greater probability compared to the models formed with only one risk factor. The revealed increase in the degree of neocortex induction during brain cerebral ischemia in mice with herpes infection is an evidence of possible connection between the two.
The aim: To determine the frequency of HSV1, HSV2, VZV, CMV, EBV, HHV6 and influenza virus detection in patients with ischemic stroke in different seasons. Materials and methods: 144 patients with ischemic stroke were examined: 78 (54.2%) women and 66 (45.8%) men, mean age of 63.1 ± 0.8 years. Detection of the herpesvirus DNA and the influenza virus RNA was performed using PCR monthly in 12 patients. Results: A manifestation of a viral infection was detected in 32 (22.2%) and virus genomes were observed in 29 (90.6%) patients. Viral infection frequency is significantly lower in summer, compared to winter-autumn; p=0.033. HSV1 and HHV6 were the most common (19 (52.8%) and 16 (44.4%)); VZV was the least common (5 (13.9%)). Influenza virus RNA was detected in 10 (27.8%) patients. In winter-autumn the frequency of HSV1, HSV2, HHV6 viruses detection is significantly higher, compared to the spring-summer (p<0.05), and the difference is almost significant for the influenza virus (p=0.060) and the EBV (p=0.060). Association of stroke occurrence with the presence of two or more types of viruses is more common in winter, compared to the summer season: 11 (30.6%) vs. 3 (8.3%), p=0.017. Conclusions: Prevention and treatment of herpesvirus infections exacerbations, in particular HSV1 and HSV2, which significantly increase in winter, compared to summer, is an important direction of stroke prevention measures in risk groups.
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